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Enhancement in Efficiency of Polymerase Chain Reaction by Silver Nano-Particles

Author Affiliations

  • 1Department of Biotechnology, University of Mumbai, Vidyanagari, Kalina, Santacruz (E), Mumbai, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (2), Pages 30-33, February,10 (2014)


This experiment evaluated the effect of silver nano-particles on the efficiency of Polymerase Chain Reactions. Silver nano-particles were prepared by chemical reduction method. The particles were analyzed by Particle Size Analyzer and UV-Vis Spectrophotometer. AFM image for the particles was taken. Different concentrations of the prepared nano-particles of median size 50.30 nm were used with other components of PCR reaction mixture and the reactions were carried out along with the control. After Agarose Gel Electrophoresis, the DNA bands were observed. The experiment showed that the prepared Silver nano-particles enhance the PCR efficiency when added at a concentration of 0.02nM.


  1. Von Wintzingerode F, G¨obel UB & Stackebrandt E, Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis, FEMS Microbiol Rev,21, 213–229 (1997)
  2. Suzuki MT & Giovannoni SJ, Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR., Appl Environ Micro,62, 625–630 (1996)
  3. Stults JR, Snoeyenbos-West O, Methe B, Lovley DR & Chandler DP, Application of the 50 fluorogenic exonuclease assay (TaqMan) for quantitative ribosomal DNA and rRNA analysis in sediments, Appl Environ Microb,67, 2781–2789 (2001)
  4. Holland PM, Abramson RD, Watson R and Gelfand DH, Detection of specific polymerase chain reaction product by utilizing the 50–30 exonuclease activity of Thermus aquaticus DNA polymerase, P Natl Acad Sci USA,88, 7276–7280 (1991)
  5. Becker S, Boger P, Oehlmann R & Ernst A, PCR bias in ecological analysis: a case study for quantitative Taq nuclease assays in analyses of microbial communities, Appl Environ Microb, 66, 4945–4953 (2000)
  6. Takai K & Horikoshi K, Rapid detection and quantification of members of the archaeal community by quantitative PCR using fluorogenic probes, Appl Environ Microb,66, 5066–5072 (2000)
  7. Banerjee M., Chongad L. and Sharma A, Structural and Optical Properties of Pure and Copper Doped NiS Nanoparticles, Research Journal of Recent Sciences, 2(ISC-2012) , 326-329 (2013)
  8. Maheswaran S., Bhuvaneshwari B., Palani G.S., Nagesh R Iyer and Kalaiselvam S, An Overview on the Influence of Nano Silica in Concrete and a Research Initiative, Research Journal of Recent Sciences, 2(ISC-2012), 17-24 (2013)
  9. Min Li, Yu-Cheng Lin, Chao-Chin Wu and Hsiao-Sheng Liu , Enhancing the efficiency of a PCR usinggoldnano-particles, Nucleic Acids Research, (2005)
  10. Binh V. Vu, Dmitri Litvinov, and Richard C. Willson, Gold Nano-particle Effects in Polymerase Chain Reaction: Favoring of Smaller Products by Polymerase Adsorption, Anal. Chem., 80, 5462–5467 (2008)
  11. Sareen Sarah J., Pillai Raji K., Chandramohanakumar N and Balagopalan M, Larvicidal Potential of Biologically Synthesised Silver Nanoparticles against Aedes Albopictus, Research Journal of Recent Sciences , 1(ISC-2011) , 52-56 (2012)
  12. Pillai Raji K., Sareen Sarah John., Toms Joseph C., Chandramohanakumar N. and Balagopalan M., Vermifugal Activity of Biofabricated Silver Nanoparticles, Research Journal of Recent Sciences, 1(ISC-2011), 47-51 (2012)
  13. Akira Ono, Shiqi Cao, Humika Togashi, Mitsuru Tashiro, Takashi Fujimoto, Tomoya Machinami, Shuji Oda, Yoko Miyake, Itaru Okamoto and Yoshiyuki Tanaka, Specific interactions between silver(I) ions and cytosine–cytosine pairs in DNA duplexes, Chem. Commun., 4825-4827 (2008)
  14. Asta ŠILEIKAIT1, Igoris PROSYEVAS, Judita PUIŠO, Algimantas JURAITIS, Asta GUOBIEN, Analysis of Silver Nanoparticles Produced by Chemical Reduction of Silver Salt Solution , ISSN 1392–1320 MATERIALS SCIENCE (MEDIAGOTYRA). 12(4)(2006)
  15. Bangale Sachin and Bamane Sambaji, Synthesis, Characterization and Hydrophilic Properties of Nanocrystalline ZnFe2O4 Oxide, Research Journal of Recent sciences, 1 (ISC-2011), 202-206 (2012)
  16. Rai M., Yadav A. and Gade A., Silver nanoparticles as a new generation of antimicrobials, Biotechnol.Adv., 27, 76-83 (2009)