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Enhancement in Efficiency of Polymerase Chain Reaction by Silver Nano-Particles

Author Affiliations

  • 1Department of Biotechnology, University of Mumbai, Vidyanagari, Kalina, Santacruz (E), Mumbai, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (2), Pages 30-33, February,10 (2014)

Abstract

This experiment evaluated the effect of silver nano-particles on the efficiency of Polymerase Chain Reactions. Silver nano-particles were prepared by chemical reduction method. The particles were analyzed by Particle Size Analyzer and UV-Vis Spectrophotometer. AFM image for the particles was taken. Different concentrations of the prepared nano-particles of median size 50.30 nm were used with other components of PCR reaction mixture and the reactions were carried out along with the control. After Agarose Gel Electrophoresis, the DNA bands were observed. The experiment showed that the prepared Silver nano-particles enhance the PCR efficiency when added at a concentration of 0.02nM.

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