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Biochemical factors, MDA levels and Antioxidant activity in Opium addicted Hamsters

Author Affiliations

  • 1Department of Biochemistry and Nutrition, Hamadan University of Medical Sciences, Hamadan, IRAN
  • 2 Research Center for Molecular Medicine, Hamadan University of and Medical Sciences, Hamadan, IRAN
  • 3 Department of Laboratory Sciences, Para-medical Faculty, University of Medical science, Kermanshah, IRAN
  • 4 Kermanshah University of Medical Sciences, Kermanshah, IRAN
  • 5 Department of Health, University of Medical science, Kermanshah, IRAN
  • 6 Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IRAN
  • 7 Department of Biology, Islamic Azad University, Borojerd Branch, Borojerd, IRAN
  • 8 Student Research Committee, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, IRAN
  • 9 Sabzevar University of Medical Sciences, Sabzevar, IRAN

Int. Res. J. Biological Sci., Volume 3, Issue (2), Pages 21-24, February,10 (2014)

Abstract

In this experiment, 12 male golden Syrian hamsters were divided into two groups containing six animalseach: Group 1 received chow diet (control); group 2 received opium (addicted). All hamsters were sacrificed after 24 hours of the final treatment (after 4four weeks), and the blood of animals were collected. Lipid profiles and liver enzymes were measured enzymatically. Atherogenic Index (AI) and LDL-C were calculated. Also, the LDL-C to HDL-C ratiowas determined.Serum super oxide dismutase (SOD) activity was measured by the method Misra and Fridovich method. The level of malondialdehyde (MDA) was evaluated by Ohkawa et al method. Catalase (CAT) and reduced glutathione (GSH) activity were measured by Aebi and Beutler E, et al. methods respectively. Amount of total cholesterol had a non-significant decreased in opium group. Serum TG and VLDL-C increased in opium group but it was not significant. Change in liver enzymes, were not significant in opium group compared to control hamster. The plasma concentration of MDA markedly increased in opium (p < 0.01) group compared to control. SOD, GSH and catalase levels also markedly reduced in opium (p <0.05) group compared to healthy hamster. In conclusion, oxidative stress is increased in opium treated animals.

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