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Determination of Crude Oil Degradation Efficiency of Glass Biofilm of Isolated Bacterium and Fungus

Author Affiliations

  • 1Loyola Centre for Research and Development, Navarangpura, Ahmedabad, Gujarat, INDIA
  • 2St. Xavier’s College, Nr. Gujarat University, Ahmedabad. Gujarat, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (2), Pages 67-69, February,10 (2014)

Abstract

The natural ecosystem has greatly affected by the spills of crude and refined oils from various oil refineries. Different physical, chemical and biochemical techniques have been applied to degrade the crude oils spills. One of such technique is use of biofilm of oil degrading microbes. The present work has focused on the use of biofilms as a mean to degrade crude oil due to some of its property which makes its more advantageous then the planktonic cells. In this experiment, microbes having potential to degrade crude oil were isolated and biofilms of these microbes were prepared on glass matrix. These films were utilized for determination of their crude oil degradation efficiency. Not only this, phase contrast microscopic analysis was carried out to determine the biofilm consistency on glass matrix, before and after degradation study. Results of the study have shown that among the isolated microbes, Bacilli has maximum potential to make a firm biofilm with glass as compare to fungal spp. In addition to that, it also has great potential to degrade 90.0% of crude oil within 22hrs.

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