9th International Science Congress (ISC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Determination of Crude Oil Degradation Efficiency of Glass Biofilm of Isolated Bacterium and Fungus

Author Affiliations

  • 1Loyola Centre for Research and Development, Navarangpura, Ahmedabad, Gujarat, INDIA
  • 2St. Xavier’s College, Nr. Gujarat University, Ahmedabad. Gujarat, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (2), Pages 67-69, February,10 (2014)


The natural ecosystem has greatly affected by the spills of crude and refined oils from various oil refineries. Different physical, chemical and biochemical techniques have been applied to degrade the crude oils spills. One of such technique is use of biofilm of oil degrading microbes. The present work has focused on the use of biofilms as a mean to degrade crude oil due to some of its property which makes its more advantageous then the planktonic cells. In this experiment, microbes having potential to degrade crude oil were isolated and biofilms of these microbes were prepared on glass matrix. These films were utilized for determination of their crude oil degradation efficiency. Not only this, phase contrast microscopic analysis was carried out to determine the biofilm consistency on glass matrix, before and after degradation study. Results of the study have shown that among the isolated microbes, Bacilli has maximum potential to make a firm biofilm with glass as compare to fungal spp. In addition to that, it also has great potential to degrade 90.0% of crude oil within 22hrs.


  1. Aguilera F., Méndez J., Pásaro E. and Laffon B., Review on the effects of exposure to spilled oils on human health, Journal of applied toxicology,30, 291-301 (2010)
  2. Dave D. and Ghaly A.E., Remediation Technologies for Marine Oil Spills: A Critical Review and Comparative Analysis, American Journal of Environmental Sciences,, 423-440 (2011)
  3. Uzoamaka G.O., Floretta T. and Florence M.O., Hydrocarbon Degradation Potentials of Indigenous Fungal isolates from petrolwum Contaminated Soils, Jounral of physical and natural Sciences,, 1-6 (2009)
  4. Turner M., Review of Offshore Oil-spill Prevention and Remediation Requirements and Practices in Newfoundland and Labrador (2010)
  5. Maruthi Y.A., Hossain K. and Thakre S., Aspergillus flavus: A potential Bioremediator for oil contaminated soils Abstract: European Journal of Sustainable Development,, 57-66 (2013)
  6. Lotfinasabasl S., Gunale V.R. and Rajurkar N.S., Assessment of Petroleum Hydrocarbon Degradation from Soil and Tarball by Fungi, Bioscience Discovery,, 186-192 (2012)
  7. Kokare C.R., Chakraborty S., Khopade A.N. and Mahadik K.R., Biofilm: Importance and applications, Indian Journal of Biotechnology,, 159-168 (2009)
  8. Kearns D.B., Chu F., Branda S.S., Kolter R. and Losick R., A master regulator for biofilm formation by Bacillus subtilis, Molecular microbiology,55, 739-49 (2005)
  9. Prakash B., Veeregowda B.M. and Krishnappa G., Biofilms: A survival strategy of bacteria, Current Science,85, 1299-1307 (2003)
  10. Orsod M., Joseph M. and Huyop F., Characterization of Exopolysaccharides Produced by Bacillus cereus and Brachybacterium sp . Isolated from Asian Sea Bass (Lates calcarifer), Malasian Journal of Microbiology,, 170-174(2012)
  11. Vijayabaskar P., Babinastarlin S., Shankar T., Sivakumar T. and Anandapandian K.T.K., Quantification and Characterization of Exopolysaccharides from Bacillus subtilis (MTCC 121), Advances in biological Research,, 71-76 (2011)
  12. Sutherland I.W., Biofilm exopolysaccharides: a strong and sticky framework, Microbiology,147, 3-9 (2001)
  13. Kwon K.K. et al., Isolation and Identification of Biofilm-Forming Marine Bacteria on Glass Surfaces in Dae-Ho Dike, Korea, The Jounral of Microbiology, 40, 260-266 (2002)