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Decolourization of Two Industrial Dyes by Bacteria from Paper and Pulp Mill Effluents

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Author Affiliations

  • 14 Department of Microbiology, University of Chittagong, Chittagong – 4331, BANGLADESH

Int. Res. J. Biological Sci., Volume 3, Issue (12), Pages 51-55, December,10 (2014)

Abstract

The effluents of paper and pulp industries are the major sources of water pollution, threatening the existence of aquatic biodiversity. So, their treatment before discharge is very crucial and biodegradation where microorganisms break down the azo bonds to form its nontoxic basic element is the most effective way. Our research work deals with the physicochemical characterization of the concerned effluent, isolation of indigenous bacterial strain and evaluation of their decolorizing efficiency. Decolorization assay was done spectrophotometrically in Mineral salt medium modified with 0.005% of the tested dyes viz. Brilliant green and Methyl violet after regular interval of incubation period (3, 5 and 7 days). All the collected effluent samples were found to characterize by high BOD, COD, TSS and TDS values. Five potential isolates viz. Alcaligenes eutrophus, Micrococcus luteus, Bacillus licheniformis, Bacillus subtilis, and Pseudomonas syringae were identified and their decolourizing capability were evaluated. Alcaligenes eutrophus Micrococcus luteus. and Pseudomonas syringae were found to decolourize around 80% of the Brilliant Green after 5 days of incubation whereas Bacillus subtilis and Bacillus licheniformis causes slightly more than 60% decolourization after the same incubation period. Bacillus licheniformis was found to be the only isolate capable of decolourizing Methyl violet with maximum of 75% after 7 days of incubation. These findings reveal the existence of indigenous dye decolourizer and signify their implementation in biotreatment of the tested dyes.

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