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Effect of Three Commonly Used Insecticides on Histomorphology and Histochemistry of ovary of an Earthworm Eudichogaster Kinneari (Stephenson)

Author Affiliations

  • 1 Department of Zoology, Govt. Girls P.G. College, Ujjain, M.P., INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (12), Pages 29-36, December,10 (2014)

Abstract

Eudichogaster kinneari were exposed to 0.6 ppm concentration of Dimethoate, 0.5 ppm concentration of Azodrin and 0.003 ppm concentration of Thiodan for 20 days to evaluate profound changes in the histomorphology and histochemistry of ovary by adding vacuolization due to congregation of ooplasm and nucleoplasm, irregular thickened cell boundary of oolema and nucleolemma, uneven stain were seen in whole structure, deterioration of ooplasm and nucleoplasm and ultimately destruction of cellular architecture of all stages of oocytes showed atrophied condition. Decreased intensity with histochemical reactions and reduced diameter of all stages of oocytes (p0.001) were observed. The present study indicates that among the three insecticides tested, thiodan is most toxic to earthworm E.kinneari, than azodrin and dimethoate respectively. The intensity of deterioration were noticed more toxic in thiodan &

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