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Toxic effects of Cadmium and Popper on Gillsurface Ultra structure of Anabas testudineus Bloch: A scanning Electron Microscopic Study

Author Affiliations

  • 1Department of Ecology and Environmental Science, Assam University, Silchar, Assam, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (11), Pages 18-22, November,10 (2014)

Abstract

The effects of a 96 hexposure to 40.898 mg L-1 Cd and 0.980 mg L -1Cu, which represented the 96 h LC50 of these two metals, on gill surface ultra structure of Anabas testudineus was studied using scanning electron microscopy. Fish gills comprise primary and secondary lamellae, which have three different cell types: epithelial pavement cells, chloride cells and mucous cells. The changes in gill surface ultra structure were characterized by fusion of adjacent secondary lamellae, oedema, disruption of gill epithelium, and excretion of large amount of mucous on lamellar surface. These alterations demonstrate the morphological as well as physiological responses of the fish when exposed to Cd and Cu. The study shows scanning electron microscopy to be useful tool for identifying morphological biomarkers on the gills of Anabas testudineus.

References

  1. Manoj K. and Padhy P.K. Oxidative stress and heavy metals: an appraisal with reference to environmental biology, Int. Res. J. Biological Sci., 2(10), 91-101 (2013)
  2. Rajiv P., Hasna A.S., Kamaraj M., Rajeshwari S. and Sankar A., Physico-chemical and microbial analysis of different river waters in Western Tamil Nadu, India. Int. Res. J. Environment Sci., 1(1), 2-6 (2012)
  3. Jezierska B. and Witeska M., Metal toxicity to fish. University of Podlasie, Siedlce (2001)
  4. Mendez-Armenta M. and Rios C., Cadmium neurotoxicity, Mini review, Environ. Toxicol. Pharmacol., 23, 350–358 (2007)
  5. Walkes M.P., Cadmium carcinogenesis in review, J. Inorg. Bichem., 241–244 (2000)
  6. Gabbianelli R., Lupidi G., Villarini M. and Falcioni G., DNA damage induced by copper in erythrocytes of gilthead sea bream Sparusaurata and mollusc, Scapharcainaequivalvis. Arch. Environ. Contam. Toxicol., 45, 350–356 (2003)
  7. Cavas T., Garanko N.N. and Arkhipchuk V.V., Induction of micronuclei and binuclei in blood, gill and liver cells of fishes subchronically exposed to cadmium chloride andcopper sulphate, Food Chem Toxicol., 43, 569–574 (2005)
  8. Fedeli D., Carloni M. and Falcioni G., Oxidative damage in trout erythrocyte in response to ‘‘in vitro’’ copper exposure, Marine Environ. Res., 69, 172–177 (2010)
  9. Nwajei G.E., Obi–Iyeke G.E. and Okwagi P., Distribution of selected trace metal in fish parts from the River Niger, Res. J. Recent Sci., 1(1), 81-84 (2012)
  10. Maina J.N., The Gas Exchangers. Structure, Function and Evolution of the Respiratory Processes, Springer, New York, 498 (1998)
  11. Wendelaar Bonga S.E. and Lock R.A.C., The osmoregulatory system, in Di Giulio, R.T. and Hinton, D.E. (Eds) : The Toxicology of Fishes, CRC Press-Taylor and Francis Group, Boca Raton, FL, 401–415 (2008)
  12. Vigliano F.A., Aleman N., Quiroga M.I. and Nieto J.M., Ultrastructural characterization of gills in juveniles Argentinian Silverside, Odontesthesbonariensis (Valenciennes 1835)
  13. (Teleostei : Atheriniformes), Anat. Histol. And Embryol., 35, 76–83 (2006)
  14. Ganguly S., Human Health benefits from fish consumption and environmental toxicity issues in fish flesh, Int. Res. J. Biological Sci., 2(9), 84-85 (2013)
  15. Kartika B., Diana A., Marsoedi and Hakim H., Effects of protein levels on the growth of climbing perch, Anabas testudineus Galam type, in Peat water, Int. Res. J. Biological Sci., 2(4), 55-58 (2013)
  16. Kirschner L.B., The mechanism of sodium chloride uptake in hyper regulating aquatic animals, J. Exp. Biol., 207, 1439–1452 (2004)
  17. Evans D.H., Piermarini P.M. and Choe K.P., The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol. Rev., 85, 97–177 (2005)
  18. Fernandes M.N. and Perna-Martins S.A., Epithelial gill cells in the armoredcatfish, Hypostomus CF. Plecostomus (Loricariidae), Revista Brasileira de Biología, 61, 69–78 (2001)
  19. Poleksic V., Lenhardt M., Jaric I., Djordjevic D., Gacic Z., Cvijanovic G. and Raskovic B., Liver, gills, and skin histopathology and heavy metal content of the Danube starlet (Acipenserruthenus Linnaeus, 1758) , Environ. Toxicol. Chem., 29(3), 515–521 (2010)
  20. Chakpram R. and Gupta A., Effects of cadmium and copper on survival and growth of Anabas testudineus, Bloch, Res. J. Chem. Environ. Sci. 2(2) (2014)
  21. Gupta S. and Gupta A., Scanning electron microscopic study of the cuticular structures on the head of Gerris Sp. (Hemiptera: Gerridae) and Cloeon Sp. (Ephemeroptera : Baetidae), Entomen., 29(1), 25-30 (2004)
  22. Thophon S., Kruatrachue M., Upatham E.S., Pokethitiyook P., Sahaphong S. and Jaritkhuan S., Histopathological alterations of white seabass Latescalcarifer, in acute and subchronic cadmium exposure. Environ. Pollut., 121, 307-320 (2003)
  23. Dang Z., Lock R.A.C. and Flik G., Wendelaar Bonga S.E., Metallothionein response in gills of Oreochromismossambicus exposed to copper in fresh water, Am. J. Physiol–Regul. Integr, Comp. Physiol., 277, 320–331 (1999)
  24. Cengiz E.I. and Unlu E., Histopathological changes in the gills of mosquito fish Gambusiaaffinis exposed to endosulfan, Bull. Environ. Contam.Toxicol., 68, 290–296 (2002)
  25. Poleksic V. and Jermic S., Effects of acute cyanide poisoning on the fish gills, Ind.Pol., 802–808 (2003)
  26. Parashar R.S. and Banerjee T.K.., Toxic impact of lethal concentration of leadnitrate on the gills of air-breathing catfish Heteropneustesfossilis (Bloch), Vet.Arch., 72, 167–183 (2002)
  27. Stouthart A.J.H.X., Spanings F.A.T., Lock R.A.C. and Wendelaar Bonga S.E., Effects of water pH on chromium toxicity to early life stages of the common carp (Cyprinuscarpio), Aquat. Toxicol., 32, 31–42 (1995)
  28. Mazon A.F., Cerqueira C.C.C. and Fernandes M.N., Gill cellular changes induced by copper exposure in the South American tropical freshwater fish Prochilodusscrofa, Environ. Res., 88A, 52–63 (2002)
  29. Monteiro S.M., Rocha E., Mancera J.M., Fontaínhas-Fernandes, A. and Sousa M. A stereological study of copper toxicity in gills of Oreochromisniloticus, Ecotox. Environ. Saf., 72, 213–223 (2009)
  30. Palaniappan P.L.RM., Selvi Sabhanayakam, Krishnakumar N. and Vadivelu M., Morphological changes due to lead exposure and the influence of DMSA on the gill tissues of the freshwater fish, Catlacatla, Food and chemical Toxicol., 46, 2440-2444 (2008)
  31. Daiane C.M.S., Sergio L.P.M., Juraci A.O. and Jorge A.D.S., Histological alterations in gills of Astyanaxaff. bimaculatus caused by acute exposition to zinc, Exp. Toxicol. pathol., 64(7-8), 861-866 (2012)
  32. Ferrari L., Eissa B.L., Ossana N.A. and Salibián A., Effects of sublethal waterborne cadmiumon gills in three teleosteanspecies : Scanning electron microscope study,Int. J. Environ. Health., 3(4), 410-426 (2009)
  33. Laurent P., Hobe H. and Dunel-Erb S., The role of environmental sodium chloriderelative to calcium in gill morphology of fresh water salmonid fish, Cell Tissue Res., 240, 675–692 (1985)
  34. Pisam M. and Rambourg A., Mitochondria-rich cells in the gill epithelium ofteleost fishes : An ultra structural approach, Int. Rev. Cytol., 130, 191–232 (1991)
  35. Saboia-Moraes S.M.T., Hernandez-Blazquez F.J., Mota D.L. and Bittencourt A.M., Mucous cell types in the branchial epithelium of the euryhaline fish Poeciliavivipara, J. Fish Biol., 49, 545–548 (1996)
  36. Mc Cahon C.P., Pascoe D. and Kavnagh M., Histochemical observations on the salmonids Salmosalar . and Samotruta L. and the ephemeropterans Baetisrhodani (Pict.) and Ecdyonurusvenosus (Fabr.) following a simulated episode of acidity in an upland stream, Hydrobiologia.,153, 3–12 (1987)
  37. Handy R.D., Eddy F.B. and Romain G., In vitro evidence for the ionoregulatory role of rainbow trout mucus in acid, acid/aluminium and zinc toxicity, J. Fish Biol., 35, 737–747 (1989)
  38. Kossakowski M.K. and Ostaszewska T., Histopathological changes in the Juvenilecarp Cyprinuscarpio, Arch. Pol. Fish., 11, 57–67 (2003)