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Effect of Desiccation of Maize Calli on the Efficiency of Transformation Prior and Post to Infection by Agrobacterium Tumefaciens

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Author Affiliations

  • 1 Dept. of Biochemistry and Bioprocess Technology, Sam Higginbottom Institute of Agriculture, Technology and Sciences, Allahabad, INDIA
  • 2Biotechnology lab., Division of Crop Improvement, Vivekanand Parvathiya Krishi Anusandhan Sansthan ,(ICAR), Almora, Uttarakhand, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (11), Pages 59-62, November,10 (2014)

Abstract

MS media containing 6-benzyl-amino-purine (3mg/ L) and picloram (10mg/L) considered for seed germination, after 10 days conspicuous nodes were splitted into two longitudinal halves then inoculated on MS callusing media having 2-4 dicholorophenoxyacetic acid (0.5mg/L ) with picloram (2.2 mg/L). Seedling derived callus of maize inbred line VQL2 was used for Agrobacterium transformation. Calluses desiccated for 1 hour prior to infection resulted in maximum greening 91.2%, whereas with 79.2% greening, calluses desiccated for 3 hours prior to infection showed highest percentage 10.4% of plant regeneration. Though greening was 6% and 12% in calluses desiccated for 2 and 3 hours respectively, prior to infection, no root and shoot regeneration was observed. Ascorbic acid was also having positive effects as 32.33% greening was observed in the calluses.

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