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Molecular Identification of Listeria Species from Vegetables Marketed in Mysore, Karnataka, India

Author Affiliations

  • 1Department of Studies in Microbiology, University of Mysore, Manasagangotri, Mysore 570 006, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (11), Pages 1-4, November,10 (2014)

Abstract

The incidence of Listeria species in vegetables marketed in Mysore city, Karnataka, India was studied. One hundred and sixty five vegetable samples marketed were analyzed for the presence of Listeria spp. Cold enrichment procedure was used for the isolation and plating was done on two selective agars PALCAM and Oxford agars. The identification of the isolates was done by biochemical characterization and confirmation by Polymerase Chain Reaction using species and genus specific primers. Three samples were found positive for Listeria spp. and they were confirmed as L. innocua and L. seeligeri.

References

  1. http://www.ehagroup.com/food-safety/fruits-vegetables (2014)
  2. Beuchat L.R., Listeria monocytogenes: Incidence on vegetables, Food Control, 7(4-5), 223-228 (1996)
  3. Khelef N., Lecuit M., Buchrieser C., Cabanes D., Dussurget O. and Cossart P., Bacteria : Firmicutes, Cyanobacteria, In M. Dworkin (Ed.), Prokaryotes A handbook on the biology of bacteria. New York: Springer, 4, 404-476, (2006)
  4. Mc Lauchlin J. and Rees C.E.D., Genus Listeria Pirie 1940a, 383AL, In Bergey’s Manual of Systematic Bacteriology, New York : Springer; The Firmicutes 2, ISBN 978-0-387-95041-9, (1997)
  5. Bertsch D., Rau J., Eugster M.R., Haug M.C., Lawson P.A., Lacroix C. and Meile L., Listeria fleischmannii sp. nov., isolated from cheese, Int. J. Syst. Evol. Micr.,63, 526-532 (2013)
  6. Halter E.L., Neuhaus K. and Scherer S., Listeria weihenstephanensis sp. nov., isolated from the water plant Lemna trisulca taken from a freshwater pond, Int. J. Syst. Evol. Micr.,63, 641-647 (2013)
  7. Liu D., Identification, subtyping and virulence determination of Listeria monocytogenes, an important foodborne pathogen, J. Med. Microbiol.,55, 645-659 (2006)
  8. Freitag N.E., Port G.C. and Miner M.D., Listeria monocytogenes - from saprophyte to intracellular pathogen, Nat. Rev. Microbiol.,, 623-628 (2009)
  9. Dhanashree B., Otta S.K., Karunasagar I., Goebel W. and Karunasagar I., Incidence of Listeria species in clinical and food samples in Mangalore, India, Food Microbiol.,20, 447- 453 (2003)
  10. Bubert A., Kohler S. and Goebel W., The homologous and heterologous regions within the iap gene allow genus- and species specific identification of Listeria spp. by Polymerase chain reaction, Appl. Environ. Microb.,58(8), 2625-2632 (1992)
  11. Bubert A., Hein I., Rauch M., Lehner A., Yoon B., Goebel W. and Wagner M., Detection and Differentiation of Listeria spp. by a Single Reaction Based on Multiplex PCR, Appl. Environ. Microb.,65(10), 4688–4692 (1999)
  12. Burnett S.L. and Beuchat L.R., Human pathogens associated with raw produce and unpasteurized juices, and difficulties in decontamination, J. Ind. Microbiol. Biot.,27(2), 104-110 (2001)
  13. Johnston L.M., Jaykus L.A., Moll D., Anciso J., Mora B. and Moe C.L., A field study of the microbiological quality of fresh produce of domestic and Mexican origin, Int. J. Food Microbio.,112(2), 83-95 (2006)
  14. Arumugaswamy R.K., Ali G.R. and Abd Hamid S.N., Prevalence of Listeria monocytogenes in foods in Malaysia, Int. J. Food Microbio.,23(1), 117-21 (1994)
  15. Abadias M., Usall J., Anguera M., Solsona C. and Viñas I., Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments Int. J. Food Microbio.,123, 121–129 (2008)
  16. Gianfranceschi M., Gattuso A., Tartaro S. and Aureli P., Incidence of Listeria monocytogenes in food and environmental samples in Italy between 1990 and 1999: Serotype distribution in food, environmental and clinical samples, Eur. J. Epidemiol., 18(10), 1001-1006 (2003)
  17. Wong H.C., Chao W.L. and Lee S.J., Incidence and characterization of Listeria monocytogenes in foods available in Taiwan, Appl.Environ. Microb.,56(10), 3101–3104 (1990)
  18. Szymczak B., Szymczak M., Sawicki W. and Dbrowski W., Anthropogenic impact on the presence of . monocytogenes in soil, fruits, and vegetables, Folia Microbiol.,59(1), 23-29 (2013)
  19. Lin C.M., Fernando S.M. and Wei C., Occurrence of Listeria monocytogenes, Salmonella spp., Escherichia coli and E. coli O157:H7 in vegetable salads, Food Control, 7(3), 135–140 (1996)
  20. Heisick J.E., Wagner D.E., Nierman M.L. and Peeler J.T., Listeria spp. found on fresh market produce, Appl. Environ. Microb., 55(8), 1925–1927 (1989)
  21. Ponniah J., Robin T., Paie M.S., Radu S., Ghazali F.M., Kqueen C.Y., Nishibuchi M., Nakaguchi Y., and Malakar P.K., Listeria monocytogenes in raw salad vegetables sold at retail level in Malaysia, Food Control,21(5), 774-778 (2010)