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Effects of Vitamins A,C and E on Growth and Colonial Morphology of Aspergillus flavus

Author Affiliations

  • 1Department of Biological Sciences, College of Science and Mathematics, MSU-Iligan Institute of Technology, Iligan City, PHILIPPINES

Int. Res. J. Biological Sci., Volume 3, Issue (10), Pages 52-59, October,10 (2014)

Abstract

Contamination of the aflatoxin-producing Aspergellus flavus occurs in several economical significant crops causing decrease in crop yield and pose health problems. This study was designed to evaluate the ability of vitamins A, C, E, and its combinations to inhibit fungal growth with corresponding colonial morphology changes. This was done by adding a specific concentration of the different vitamins to a culture media and a single point inoculation was employed. It was then incubated for seven (7) days after which growth inhibition, total dry weights, and radial growth were obtained. Results of showed that both growth and mycelia biomass of A.flavus were affected by the vitamins used and its combinations. Among all the vitamins used, at higher concentrations, vitamin C (ascorbate) has complete inhibitory effects to the growth. Decreasing concentrations of thse vitamins in the medium led to an increasing amount of fungal growth as well as increasing fungal biomass and radial growth rates. It was observed that vitamin A, C, and E inhibit fungal growth in a similar way though its combinations exhibited lesser inhibitory effects. In addition, significant concentration-dependent inhibition of growth, mycelia biomass and radial growth rate was evident for selected vitamins. Thus, vitamins A, C, and E can be used to control fungal growth in important crops and processed agricultural products.

References

  1. Fakhoury A.M. and Woloshuk C.P., Inhibition of growth of Aspergellus flavus and fungal -Amylase by a Lectin-like protein from Lablap purpureus, Phytopathology,8, 955-961 (2001)
  2. European Food Safety Authority, Opinion of the Scientific Panel on Contaminants in the Food Chain on a request from the European Commission related to the potential increase of consumer health risk by a possible of the existing maximum levels for aflatoxins in almonds, hazelnuts and pistachios and derived products, The EFSA Journal,446, 1-127 (2007)
  3. Onilude A.A., Fagade O.E., Bello M.M. and Fadahunsi I.F., Inhibition of aflatoxin-producing aspergilla by lactic acid bacteria isolates from indigenously fermented cereal gruels, Biotechnology,, 1404-1408 (2005)
  4. Coulumbe R.A., Aflatoxin, In: Sharma RP and SALUNKHE DK (Eds), Mycotoxins and Phytoalexins, London: CRC Press, 103-144 (1991)
  5. Lane K, Minimizing aflatoxin on tobacco as a value-added American crop, retrieved from http://govinfo.library.unt.edu/tobacco/disc/disc18.htm., (1999)
  6. Chipley J.R. and Uraih N., Inhibition of Aspergillus growth and aflatoxin release by derivatives of benzoic acid, Appl.Environ. Microbiology, 40, 352-357 (1980)
  7. Norton R., Effect of Carotenoids on Aflatoxin B1 synthesis by A.flavus, Phytopathology,87, 814-821 (1997)
  8. Gradelet S.A., Le Bon A.M., Berges R., Suschelet M., and Astorg P., Dietary carotenoids inhibit aflatoxin B1-induced liver preneoplastic foci and DNA damage in the rat: Role of the Modulation of Aflatoxin B1 Metabolism, Carcinogenesis,19, 403-411 (1998)
  9. Peto R., Doll R., Buckley J.D., and Sporn M.B., Can dietary beta-carotene materially reduce human cancer rates?, Nature,290, 201-209 (1981)
  10. Somayeh B. and Mohammad F., Vitamin C can reduce toxic effects of Nano Zinc Oxide, International Research Journal of Biological Sciences,(3), 65-70 (2014)
  11. Georgiou C.D., and Zees A., Lipofuscins and Sclerotial Differentiation in Phytopathogenic fungi, Mycopathologia,153, 203-208 (2001)
  12. Peterscu S.A., Hulea A.S., Stan R., Avram D., and Herlea V., A yeast strain that uses D-galacturonic acid as a substrate for L-ascorbic acid biosynthesis, Biotech Lett, 14, 1-6 (1992)
  13. Spicket M.C., Smirnoff N., Pitt R.A., The biosynthesis of erythroascorbate in Saccharomyces cerevisiae and its role as an antioxidant, Free Rad Biol Med., 28, 183-192 (2000)
  14. Hansberg W., and Aguirre J., Hyperoxidant states cause microbial cell differentiation by cell isolation from dioxygen, J.Theor.Biol,142, 201-221 (1990)
  15. Elad Y., The use of antioxidants (Free radical scavengers) to control gray mold (Botrytis cinerea) and white mould (Sclerotinia sclerotiorum) in various crops, Plant Pathology,41, 417-426 (1992)
  16. Georgiou C.D., Zervoudakis G., Petropoulou K.P., Ascorbic acid might play a role in the sclerotial differentiation of Sclerotium rolfsii, Mycologia, 95, 308-316 (2003)
  17. Kumar S., and Prasad G., Efficacy of medicinal plant Andrographis peniculata) extract on aflatoxin production and growth of Aspergillus flavus, Lett. Appl. Microbiol., 15, 131 (1992)
  18. Nair A., and Verma R.J., Vitamin E ameliorates aflatoxin-induced biochemical changes in testis of mice, Asian J Androl,, 305-309 (2001)
  19. Cleveland T.E., Bhatnagar D., Foell C.J., McCormick S.P., Conversion of a new metabolite to aflatoxin B2 by Aspergillus parasiticus, Appl Environ Microbiol, 53, 2804-2807 (1987)
  20. Hamre B., Waagbo R., Berge R., Lie O., Vitamins C and E interact in juvenile Atlantic Salmon (Salmon salar, L.), Free Rad Biol Med,22, 137-149 (1997)
  21. McCay B.P., Vitamin E: Interactions with free radicals and ascorbic acid, Annu Rev Nutr.,, 323-340 (1985)
  22. Wright R.J., Colby D.H., Miles R.P., Cytosolic factors which affect microsomal lipid peroxidation in lung and liver, Arch Biochem Biophys, 206, 296-304 (1981)
  23. Bennett J.W. and Papa K.E., The aflatoxigenic Aspergillus, In:Ingram DS, Williams PA (Eds) Genetics of Plant Pathogenic Fungi, London Academic, 264-280(1988)