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Bioactivity of Endophytic Fungus Colletotrichum gloeosporioides Isolated from Phlogacanthus thyrsiflorus Nees

Author Affiliations

  • 1Department of Life Sciences, Manipur University, Manipur-795003, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (10), Pages 97-99, October,10 (2014)

Abstract

To study antibacterial, antioxidant and total phenolic content of Colletotrichum gloeosporioides an endophytic fungus isolated from Phlogacanthus thyrsiflorus Nees. Fungal endophytes were isolated from Phlogacanthus thyrsiflorus Nees collected from Imphal West, Manipur. Morphological and molecular identification was done for the potential isolate. Extract from fermentation broth of Colletotrichum gloeosporioides were checked for antibacterial activity, antioxidant activity and total phenolic content. Staphylococcus aureus (ATCC25923), Enterococcus faecalis (ATCC29212), Escherichia coli (ATCC25922) and Pseudomonas aeruginosa (ATCC27853) were used for testing antibacterial activity. The crude extract showed an effective antibacterial activity against S. aureus with a zone of inhibition of 13.331.52mm. The crude extract of an endophytic fungus Colletotrichum gloeosporioides was studied for total phenolic content and antioxidant activity. Free radical scavenging activityof the extract showed 90%. Total phenolic content is 18.30.36mg gallic acid/g of extract. This report of antibacterial and antioxidant activity of endophytic fungus Colletotrichum gloeosporioides residing in Phlogacanthus thyrsiflorus Nees is presented for the first time in this work. The findings indicate that the metabolite of endophytic fungus Colletotrichum gloeosporioides is a potent source of bioactive compounds. Further study is needed for identification of the bioactive compounds.

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