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Evaluating the Potential Cytotoxic Activity of Acmella grandiflora Flower and Whole Plant using Brine Shrimp Lethality Test

Author Affiliations

  • 1Department of Biological Sciences, MSU-Iligan Institute of Technology, Iligan City, PHILIPPINES
  • 2Department of Chemistry, MSU-Iligan Institute of Technology, Iligan City, PHILIPPINES
  • 3MSU-Malabang Community High School, Malabang, Lanao del Sur, PHILIPPINES

Int. Res. J. Biological Sci., Volume 3, Issue (10), Pages 90-92, October,10 (2014)


The use of medicinal plants among indigenous people in the Philippines draws attention from the scientific community. The Department of Science and Technology in the Philippines identifies this area as a priority for research. Acmella grandiflora of Asteraceae family is known to contain important biocomponents such as spilanthol, acmellonate, tannins, flavonoids, and phenolic compounds. This plant is traditionally used to relieve toothache and also as stomachic, stimulant, and antidiarrheal. This research was done to evaluate the potential cytotoxic activity of Acmella grandiflora using the Brine Shrimp lethality Test (BSLT). The effectivity of the ethanol extract was assessed by comparing it with the decoction and 50:50 ethanol-water mixture extract. The results showed that the extraction by ethanol is the best method of extraction. The ethanol extract (WE) of the whole plant ha d an acute LC50 of 141.25g/mL and the ethanol extract (FE) of the flowers exhibited the highest chronic LC50 value at 28.84 g/mL. Results indicate that A. grandiflora possesses potential bioactive phytochemicals worthy of further investigation specifically on its anaesthetic/sedative effect.


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