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Appraisal of in vitro Antioxidant prospective of Premna corymbosa

Author Affiliations

  • 1Research and Development Centre, Bharathiar University, Coimbatore – 641 046, INDIA
  • 2PG and Research Department of Biochemistry, Rajah Serfoji Government College (A), Thanjavur – 613005, Tamilnadu, INDIA
  • 3Armats Bioproducts Unit, Armats Biotek Pvt Ltd., Guindy, Chennai 600 032, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (10), Pages 70-75, October,10 (2014)

Abstract

Medicinal plants grip decisive liability in enhancement of individual around planet. Hi-tech explorations of medicinal plants have been instigated in copious parts of our nation as of their hand-outs to health care. In numerous bedlam, the free radical intervened injure may play an indispensable role. Free radical speculation has drastically enthused nosiness in thwarting human ailments incorporating diabetes, atherosclerosis, stroke, and rheumatoid arthritis. Antioxidants can expire or hold up the oxidation procedure by scavenging free radicals. Considering the veracity, the contemporary exploration was predestined to appraise antioxidant prospective of methanol leaf extract from Premna corymbosa. Antioxidant bustle resoluted by in vitro ordeal comprising DPPH radical foraging, hydroxyl radical foraging activity, metal chelating ability and total antioxidant activity. From outcome, extract exemplified momentous antioxidant bustle with an IC50 of 58µg/ml in DPPH foraging and 865µg/ml in hydroxyl scavenging assays. IC50 of extort inmetalchelating and total antioxidant bustle established to be 623 µg/ml and 140g/ml. Results enunciated pledging antioxidant prospective of methanol leaf extort from Premna corymbosa.

References

  1. Cuzzocrea S., Riley D.P., Caputi A.P. and Salvemini D., Antioxidant therapy: A new pharmacological approach in shock, inflammation and ischemia/reperfusion injury, Pharmacol. Rev., 53, 135-159 (2001)
  2. Shekhar H.U., Goto M., Watanabe J., Konishide-Mikami I., Bari ML., Takano-Ishikawa Y., Multi food functionalities of Kalmi Shak (Ipomoea aquatica) grown in Bangladesh, AgricFoodAnalBacteriol ., 1(1), 24-32 (2011)
  3. Subramanian L.J., Zuraini Z. and Sasidharan S., Phytochemicals screening: DPPH free radical scavenging and xanthine oxidaseinhibitiory activities of Cassia fistula seeds extract, J. Med. Plants Res., 5(10),1941-1947 (2011)
  4. Gilbert D.L., Fifty years of radical ideas, Ann NY Acad Sci; 899,1(2000)
  5. Lipinski B., Pathophysiology of oxidative stress in diabetes mellitus., J Diabetes Complications15,203-10( 2001)
  6. Baris O., Gulluce M., Sahin F., Ozer H., Kilic H., Ozkan H., Sokmen M. and Ozbek T., Biological activities of the essential oil and methanol extract of Achillea Biebersteinii Afan. (Asteraceae), Turkey Journal of Biology 30, 65-73 (2006)
  7. Nadeem Ahmad Siddique., Mohd Mujeeb., Abdul Kalam Najmi and Mohd Akram, Evaluation of antioxidant activity, quantitative estimation of phenols and flavonoids in different parts of Aegle marmelos, African Journal of Plant Science, 4 (1), 001-005 (2010)
  8. Kashikar V.S. and Kotkar Tejaswita, Indigenous remedies for diabetes mellitus, Int J Pharm Pharm Sci 3 (3), 22-29 (2011)
  9. Kapoor L.D., CRC Handbook of Ayurvedic Medicinal Plants, Herbal Reference Library, CRC Press: Boca Raton London, New York Washington, 70 (2001)
  10. Koleva I.I., Van Beek T.A., Linssen J.P., deGroot A. and Evstatieva L.N., Screening of plant extracts for antioxidant activity: A comparative study on three testing methods, Phytochem. Anal, 13, 8–17 (2002)
  11. Balestrin L., Dias J.F.G., Miguel O.G., Dall’Stella D.S.G. and Miguel M.D., Contribuiçăo ao estudo fitoquímico de Dorstenia multiformis Miquel (Moraceae) comabordagem em atividade antioxidante (in Portuguese), Rev. Bras. Farmacogn, 18,230–235 (2008)
  12. Mensor L.L., Menezes F.S., Leitao G.G., Reis A.S., dos Santos T.C. and Coube C.S, Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method, Phytother. Res, 15,127-130. (2001)
  13. Subhendu S., Mishra1 K.K., Patel. , Navdeep Raghuwanshi., Ashish Pathak Prangya Parimita Panda ., Kundlik Girhepunje and Ch. Niranjan Patro, Screening of ten indian medicinal plant extracts for antioxidant activity. Annals of Biological Research , 2 (1) ,162-170 ( 2011)
  14. Lim Y.Y., Lim T.T. and Tee J.J, Antioxidant properties of several tropical fruits: A comparative study. FoodChemistry, 103, 1003–1008 (2007)
  15. Nuno Rainha., Elisabete Lima., José Baptista and Carolina Rodrigues, Antioxidant properties, total phenolic, total carotenoid and chlorophyll content of anatomical parts of Hypericum foliosum, Journal of Medicinal Plants Research, 5(10), 1930-1940 (2011)
  16. Umamaheswari M. and Chatterjee T. K, In vitro antioxidant activities of the fractions of Coccinia grandis leaf extract, Afr J Tradit Complement Altern Med, 5(1), 61-73(2008)
  17. Singh R.P., Murthy K.N.C. and Jayaprakasha G.K, Studies on antioxidant activity of pomegranate (Punicagranatum) peel and seed extracts using in vitro models, J Agric Food Chem, 50, 81–86 (2002)
  18. Sukanya S. L., Sudisha J., Hariprasad P., Niranjana S. R., Prakash H. S. and Fathima S. K, Antimicrobial activity of leaf extracts of Indian medicinal plants against clinical and phytopathogenic bacteria. African Journal of Biotechnology, 8 (23), 6677-6682 (2009)
  19. Veerapur V. P., Prabhakar K. R., Parihar V. K., Kandadi M. R., Ramakrishana S., Mishra B., Satish Rao S. B., Srinivasan K. K., Priyadarsini K. I. and Unnikrishnan M. K,. Ficus racemosa stem bark extract: a potent antioxidant and a probable natural radioprotector. Evid. Based Complement Alternat. Med, 317-324 (2009)
  20. Kaviarasan S., Naik G.H., Gangabhagirathi R., Anuradha C.V. and Priyadarsini K.I, In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum graecum) seeds, Food Chem., 103, 31–37(2007)
  21. Nur A. M. D., Nusrat, J. B., Rafiquzzaman, M. D, Review on in vivo and in vitro methods evaluation of antioxidant activity, Saudi Pharmaceutical Journal, 21, 143-152 (2013)
  22. Li H., Wang X., Li P., Li Y. and Wang H, Comparative study of antioxidant activity of Grape (Vitis vinifera) seed powder assessed by different methods, Journal of Food and Drug Analysis, 16(6), 67-73 (2008)
  23. Harsh M, Textbook of Pathology. 6th edition. Jaypee Brothers medical publishers, New Delhi, India, 21-34 (2010)
  24. EbrahimzadehM.A., Nabavi S.M., Nabavi S.F,Correlation between the in vitro iron chelating activity and poly phenol and flavonoid contents of some medicinal plants, Pakistan J Biol Sci12, 934-938 (2009)
  25. Bush A.I., The metallobiology of Alzheimer’s diseases, Trends in Neurosciences, 26, 207-214 (2003)
  26. Varadarajan S., Yatin S., Aksennova M. and Butterfield D.A, Review: Alzheimer’s amyloid peptide-associated free radical oxidative stress and neurotoxicity, J. of Stru. Biol, 130, 184-208 (2000)
  27. Baig H., Dildar A., Saman Z., Muhammad I. and Muhammad N. A, In vitro evaluation of antioxidant properties of different solvent extracts of Rumex acetosella leaves, Oriental Journal of Chemistry, 27(4), 1509-1516(2011)
  28. Kumaran K, Estimation of total flavonoid content in propolis by two complementary colorimetric methods, J of Food and Drug Analysis, 10,178–182 (2007)