Looking for isoforms of enzymes related to in vitro morphogenesis in Nicotiana tabacum L.

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Looking for isoforms of enzymes related to in vitro morphogenesis in Nicotiana tabacum L.

Author Affiliations

¹Division of Plant Biology, Bose Institute, 93/1 APC Road, Kolkata – 700009, INDIA

Int. Res. J. Biological Sci., Volume 3, Issue (1), Pages 11-16, January,10 (2014)

Abstract

Peroxidase (PRX, E.C.1.11.1.7), Esterase (EST, E.C.3.1.1.1), Acid Phosphatase (ACP, E.C.3.1.3.2), -Amylase (E.C.3.2.1.1) and Malate Dehydrogenase (MDH, EC 1.1.1.37) enzymes were studied through native activity gel electrophoresis in three in vitro developmental stages (non regenerating callus, regenerating callus and leaves of regenerated plantlets) of tobacco (Nicotiana tabacum L.) to look for up- or down regulation of specific isoforms by densitometry scanning to correlate with changing developmental stage. Certain isoforms of Peroxidase, Esterase and Acid Phosphatase, were found to be either up- or down regulated in the course of dedifferentiation, while two other enzymes, viz. -Amylase and Malate Dehydrogenase showed little alteration in activity in the three developmental stages under study.

References

Georg-Kraemer J.E. and Cavalli S.S., Structural and regulatory differences in Amylase isoenzymes in germinating Brazilian barley cultivars, Genetics and Molecular Biology, 26, 69-75 (2003)
Bouche N. and Bouchez D., Arabidopsis gene knockout: phenotypes wanted, Current Opinion Plant Biol., 4, 111-117 (2001)
Marhavy P., Bielach A., Abas L., Abuzeineh A., Duclercq J., Tanaka H., Pa_rezova M., Petra´sek J., Ji_r´ Friml, J., Kleine-Vehn J., and Benkova´ E., Cytokinin modulates endocytic Trafficking of PIN1 auxin efflux carrier to control plant organogenesis, Dev. Cell, 21, 796–804 (2011)
Holtorf H., Guitton M.C. and Reski R., Plant Functional Genomics, Naturwissenschaften, 89, 235-249 DOI 10.1007/s00114-002-0321-3 (2002)
Gamborg O.L. and Phillips G.C., Plant cell, tissue and organ culture – fundamental methods, Heidelberg, New York, Springer-Verlag (1995)
Murashige T. and Skoog F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 15, 473 – 497 (1962)
Wetter L. and Dyck, J. Isozyme analysis of cultured cells and somatic hybrids. In: Evans, D.A., Sharp, W.R., Ammirato, P.V. and Yamada, Y., (eds.) Handbook of Plant Cell Culture, 1, Macmillam Publishing Co., NY, 607–627 (1983)
Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein measurements with the folin phenol reagent. J. Biol. Chem., 193, 265–275 (1951)
Laemmli U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature,227, 680-685 (1970)
Das S. and Mukherjee K.K., Morphological and biochemical investigations on Ipomoea seedlings and their species interrelationships, Ann. Bot., 79, 565-571 (1997)
Gangopadhyay G., Bandyopadhyay T., Modak B.K., Wongpornchai S., Mukherjee K.K., Micropropagation of Indian pandan (Pandanus amaryllifolius Roxb.), a rich source of 2-acetyl-1-pyrroline, Curr. Sc., 87, 1589-1592 (2004)
Roy S.K., Gangopadhyay G., Bandyopadhyay T., Modak B.K., Datta S. and Mukherjee K.K., Enhancement of in vitro micro corm production in Gladiolus using alternative matrix, African J. Biotech., , 1204-1209 (2006)
Kay L.E. and Basile D.V., Specific Peroxidase isoenzymes are correlated with organogenesis, Plant Physiol., 84, 99-105 (1987)
Krsnik-Rasol M., Peroxidase as a developmental marker in plant tissue culture, International J. Developmental Biol., 35, 259-263 (1991)
Marimuthu J. and Antonisamy, A., Somaclonal variation studies on Phyllanthusamarus Schum and Thonn, Iranian J. Biotech., , 240-245 (2007)
Carrillo-Castañeda G. and Mata A., Succession of Esterase and Peroxidase Isozymes associated with the in vitroSugarcane tissue dedifferentiation and shoot induction, Biotecnología Aplicada, 17, 225-230 (2000)
Panigrahi J., Behera M., Maharana S. and Mishra R.R., Biomolecular changes during in vitro organogenesis of Asteracantha longifolia (L.) Nees – A medicinal herb, Indian J. Experimental Biology, 45, 911 – 919 (2007)
Duff, S.M.G., Sarath, G. and Plaxton, W.C., The role of Acid Phosphatases in plant phosphorus metabolism, Physiol. Plant., 90, 791-800 (1994)
Ding, Y. and Ma, Q.H., Characterization of a cytosolic malate dehydrogenase cDNA which encodes an isozyme toward oxaloacetate reduction in wheat, Biochimie, 86, 509-518 (2004)

[ref1] Georg-Kraemer J.E. and Cavalli S.S., Structural and regulatory differences in Amylase isoenzymes in germinating Brazilian barley cultivars, Genetics and Molecular Biology, 26, 69-75 (2003)

[ref2] Bouche N. and Bouchez D., Arabidopsis gene knockout: phenotypes wanted, Current Opinion Plant Biol., 4, 111-117 (2001)

[ref3] Marhavy P., Bielach A., Abas L., Abuzeineh A., Duclercq J., Tanaka H., Pa_rezova M., Petra´sek J., Ji_r´ Friml, J., Kleine-Vehn J., and Benkova´ E., Cytokinin modulates endocytic Trafficking of PIN1 auxin efflux carrier to control plant organogenesis, Dev. Cell, 21, 796–804 (2011)

[ref4] Holtorf H., Guitton M.C. and Reski R., Plant Functional Genomics, Naturwissenschaften, 89, 235-249 DOI 10.1007/s00114-002-0321-3 (2002)

[ref5] Gamborg O.L. and Phillips G.C., Plant cell, tissue and organ culture – fundamental methods, Heidelberg, New York, Springer-Verlag (1995)

[ref6] Murashige T. and Skoog F., A revised medium for rapid growth and bioassays with tobacco tissue cultures, Physiol. Plant., 15, 473 – 497 (1962)

[ref7] Wetter L. and Dyck, J. Isozyme analysis of cultured cells and somatic hybrids. In: Evans, D.A., Sharp, W.R., Ammirato, P.V. and Yamada, Y., (eds.) Handbook of Plant Cell Culture, 1, Macmillam Publishing Co., NY, 607–627 (1983)

[ref8] Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein measurements with the folin phenol reagent. J. Biol. Chem., 193, 265–275 (1951)

[ref9] Laemmli U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature,227, 680-685 (1970)

[ref10] Das S. and Mukherjee K.K., Morphological and biochemical investigations on Ipomoea seedlings and their species interrelationships, Ann. Bot., 79, 565-571 (1997)

[ref11] Gangopadhyay G., Bandyopadhyay T., Modak B.K., Wongpornchai S., Mukherjee K.K., Micropropagation of Indian pandan (Pandanus amaryllifolius Roxb.), a rich source of 2-acetyl-1-pyrroline, Curr. Sc., 87, 1589-1592 (2004)

[ref12] Roy S.K., Gangopadhyay G., Bandyopadhyay T., Modak B.K., Datta S. and Mukherjee K.K., Enhancement of in vitro micro corm production in Gladiolus using alternative matrix, African J. Biotech., , 1204-1209 (2006)

[ref13] Kay L.E. and Basile D.V., Specific Peroxidase isoenzymes are correlated with organogenesis, Plant Physiol., 84, 99-105 (1987)

[ref14] Krsnik-Rasol M., Peroxidase as a developmental marker in plant tissue culture, International J. Developmental Biol., 35, 259-263 (1991)

[ref15] Marimuthu J. and Antonisamy, A., Somaclonal variation studies on Phyllanthusamarus Schum and Thonn, Iranian J. Biotech., , 240-245 (2007)

[ref16] Carrillo-Castañeda G. and Mata A., Succession of Esterase and Peroxidase Isozymes associated with the in vitroSugarcane tissue dedifferentiation and shoot induction, Biotecnología Aplicada, 17, 225-230 (2000)

[ref17] Panigrahi J., Behera M., Maharana S. and Mishra R.R., Biomolecular changes during in vitro organogenesis of Asteracantha longifolia (L.) Nees – A medicinal herb, Indian J. Experimental Biology, 45, 911 – 919 (2007)

[ref18] Duff, S.M.G., Sarath, G. and Plaxton, W.C., The role of Acid Phosphatases in plant phosphorus metabolism, Physiol. Plant., 90, 791-800 (1994)

[ref19] Ding, Y. and Ma, Q.H., Characterization of a cytosolic malate dehydrogenase cDNA which encodes an isozyme toward oxaloacetate reduction in wheat, Biochimie, 86, 509-518 (2004)