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Assessment of Parameters Influencing Rice Straw Associated Mycelial Growth of Pleurotus ostreatus MTCC 142 and a Wild Isolate of Pleurotus ostreatus

Author Affiliations

  • 1Dept. of Microbiology, Karpagam University, Coimbatore-641021, Tamil Nadu, INDIA
  • 2 Dept. of Microbiology, Genohelix Biolabs, CASB, Jain University, Bangalore-560019, Karnataka, INDIA
  • 3 Dept. of Microbial Biotechnology, Bharathiar University, Coimbatore-641046, Tamil Nadu, INDIA
  • 4

Int. Res. J. Biological Sci., Volume 2, Issue (9), Pages 15-21, September,10 (2013)

Abstract

Pleurotus ostreatus, commonly known as the oyster mushroom, is a medicinally and biotechnologically important edible mushroom. The present study focuses on assessment of various parameters which may influence the optimum mycelial growth of Pleurotus ostreatus MTCC 142 and a wild isolate of Pleurotus ostreatus. Solid-state cultivation of the mushroom was carried out using rice straw as the substrate. Indirect methods of measurement such as cellulolytic activity and total protein content were used to determine the level of rice straw associatedmycelial growth. In case of Pleurotus ostreatus MTCC 142 the highest protein yield was recorded in rice straw supplemented with 1% (w/v) potato starch and 0.5% (w/v) peptone, with an initial moisture ratio of 1:10, whereas, the highest cellulase production was associated with rice straw supplemented with 1% (w/v) lactose and 0.5% (w/v) tryptone, with an initial moisture ratio of 1:5, when inoculated with one mycelium plug. For the wild isolate of Pleurotus ostreatus the highest protein content and cellulase activity were recorded in the substrate supplemented with 1% (w/v) lactose and 0.5% (w/v) beef extract, with an initial moisture ratio of 1:10, when inoculated with two mycelial plugs.

References

  1. Asha-Augustine, Imelda-Joseph and Raj R.P., Biomass estimation of Aspergillus niger 4 a mangrove fungal isolate and A. oryzae NCIM1212 in solid-state fermentation, J Mar. Biol. Ass. India48, 139-146 (2006)
  2. Pandey A., Selvakumar P., Soccol C.R. and Nigam P., Solid state fermentation for the production of industrial enzymes, Curr. Sci.77, 149-162 (1999)
  3. Viccini G., Mitchell D.A., Boit S.D., Gern J.C., da Rosa A.S., Costa R.M., Dalsenter F.D.H., von Meien O.F. and Krieger N., Analysis of growth kinetic profiles in solid-state fermentation,Food Technol. Biotechnol., 39, 271-294 (2001)
  4. Cohen R., Persky L. and Hadar Y., Biotechnological applications and potential of wood degrading mushrooms of the genus Pleurotus, Appl. Microbial. Biotechnol. Rev., 58, 582-594 (2002)
  5. Hernādez D., Sānchez J.E. and Yamasaki K., A simple procedure for preparing substrate for Pleurotusostreatuscultivation, Bioresour. Technol., 90, 145-150 (2003)
  6. Abo-State M.A.M., Khatab O., Abo-El N.A. and Mahmoud B. Factors affecting laccase production by Pleurotus ostreatus and Pleurotus sajor-cajuWorld Appl. Sci. J, 14, 1607-1619 (2011)
  7. Bhatti M.I., Jiskani M.M., Wagan K.H., Pathan M.A. and Magsi M.R., Growth, development and yield of oyster mushroom, Pleurotus ostreatus (Jacq. Ex. Fr.) Kummer as affected by different spawn rates, Pak. J Bot., 39, 2685-2692 (2007)
  8. Banerjee U.C., Chisti Y. and Moo-Young M., Spectrophotometric determination of mycelial biomass, Biotechnol. Tech. 7, 313-316 (1993)
  9. Raimbault M., General and microbiological aspects of solid substrate fermentation, Electron. J Biotechn., , 1-15 (1998)
  10. Ooijkaas L.P., Tramper J. and Buitelaar R.M., Biomass estimation of Coniothyrium minitansin solid- state fermentation, Enzyme Microb. Technol.22, 480-486 (1998)
  11. Hashim A.J., Determination of optimal conditions for laccase production by Pleurotus ostreatus using sawdust as solid medium and its use in phenol degradation, J Baghdad for Sci., , 491-499 (2012)
  12. Junior N.M., Asai T., Capelari M. and Paccola-Meirelles L.D., Morphological and molecular identification of four Brazilian commercial isolates of Pleurotus spp. and cultivation on corncob, Braz. Arch. Biol. Technol., 53, 397-408 (2010)
  13. Sobieralski K., Siwulski M., Sas-Golak I., Makowski J. and Kotliska T., Mycelium growth and yield of wild strains of Pleurotus ostreatus (Jacq.:Fr.) Quel. cultivated on waste materials from the textile industry,Folia Hort., 23, 67-71 (2011)
  14. Bhattacharya S., Bhardwaj S., Das A. and Anand S., Utilization of sugarcane bagasse for solid- state fermentation and characterization of - amylase from Aspergillus flavus isolated from Muthupettai mangrove, Tamil Nadu, India, Aust. J Basic & Appl. Sci.,, 1012-1022 (2011)
  15. Miller G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem., 31, 426-428 (1959)
  16. Das A., Bhattacharya S., Roopa K.S. and Yashoda S.S., Microbial utilization of agronomic wastes for cellulase production by Aspergillus niger and Trichoderma viride using solid-state fermentation, Dyn. Biochem. Process. Biotech. Mol. Biol., 5, 18-22 (2011)
  17. Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein measurement with the folin phenol reagent, J Biol. Chem., 193, 265-275 (1951)
  18. Mane V.P., Patil S.S., Syed A.A. and Baig M.M.V. Bioconversion of low quality lignocellulosic agricultural waste into edible protein by Pleurotus sajor-caju (Fr.) Singer, J Zhejiang Univ. Sci., , 745-751 (2007)
  19. Akyüz M. and Kirba S., Nutritive value of wild edible and cultured mushrooms, Turk. J Biol., 34, 97-102 (2010)
  20. Agrahar-Murugkar D. and Subbulakshmi G., Nutritional value of edible wild mushrooms collected from the Khasi Hills of Meghalaya, Food Chem., 89, 599-603 (2005)
  21. Badshah N., Ur-Rehman N. and Wahid M., Yield and quality of mushrooms grown on different substrates, Sarhad J Agriculture, , 631-635 (1992)
  22. Patra A.K. and Pani B.K., Yield response of different species of oyster mushroom (Pleurotus) to paddy straw, Current Agril. Res., Supplement , 11-14 (1995)
  23. Mathew G.M., Sukumaran R.K., Singhania R.R. and Pandey A., Progress in research on fungal cellulases for lignocellulose degradation, J Sci. Ind. Res., 67, 898-907 (2008)
  24. Wang D.I.C., Cooney C.L., Demain A.L., Dunnil P., Humphrey A.E. and Lilly M.D., Fermentation and Enzyme Technology, John Wiley and Sons, New York, USA (1979)
  25. Mikiashvili N., Wasser S.P., Nevo E. and Elisashvili V., Effects of carbon and nitrogen sources on Pleurotus ostreatus ligninolytic enzyme activity, World J Microbiol. Biotechnol., 22, 999-1002 (2006)
  26. Das A., Bhattacharya S., Reddy N.V. and Sundara R.S., Improved production and purification of pectinase from Streptomyces sp. GHBA10 isolated from Valapattanam mangrove habitat, Kerala, India, Int. Res. J Biological Sci.,, 16-22 (2013)
  27. Jadhav D.M. and Gawai D.U., Effect of different nutrient sources on biomass production of phylloplane yeast Aureobasidium pullulans (De Bary), Int. Res. J Biological Sci.,, 85-87 (2012)
  28. Ramachandran S., Patel A.K., Nampoothiri K.M., Chandran S., Szakacs G., Soccol C.R. and Pandey A., Alpha amylase from a fungal culture grown on oil cakes and its properties, Braz. Arch. Biol. Techn., 47, 309-317 (2004)
  29. Schimel J.P., Gulledge J.M., Clein-Curley J.S., Lindstrom J.E. and Braddock J.F., Moisture effects on microbial activity and community structure in decomposing birch litter in the Alaskan taiga, Soil Biol. Biochem., 31, 831-838 (1999)
  30. Patil S.S., Ahmed S.A., Telang S.M. and Baig M.M.V., The nutritional value of Pleurotus ostreatus (Jacq.:Fr.) Kumm cultivated on different lignocellulosic agrowastes, Innovat. Rom. Food. Biotechnol., , 66-76 (2010)
  31. Maher M.J., Spent mushroom compost (SMC) as a nutrient in peat based potting substrates. In: Maher M.J. (ed) Science and Cultivation of Edible Fungi, Balkema, Rotterdam, Holland, 645-650 (1991)