9th International Science Congress (ISC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Assessment of Bone Loss in Postmenopausal Women by Evaluation of Urinary Hydroxyproline and Serum Status of Osteocalcin

Author Affiliations

  • 1Department of Biochemistry, College of Dental Science and Hospital, Rau, INDIA
  • 2 Department of Biochemistry, Govt. Holkar Science College, Indore, INDIA
  • 3 Department of Biochemistry, MGM Medical College, Indore, INDIA
  • 4

Int. Res. J. Biological Sci., Volume 2, Issue (9), Pages 11-14, September,10 (2013)

Abstract

Bones are in a dynamic state throughout the life. Bone metabolism is a continuous process which maintains a balance between the resorption of old and injured bone and the formation of new bone under. This continuous degradation and formation of bone is termed as bone remodeling or bone turnover, a process that occurs throughout the life. The balance between bone degradation and bone formation is altered due to aging and cessation of the menstrual cycle in women. The present study aimed at estimation of the two important markers of bone turnover, serum osteocalcin and urinary hydroxyproline in postmenopausal women and also to probe into the role of these both in assessing the altered bone metabolism. 100 women were included in the study out of which 50 were premenopausal who served as the control and 50 were postmenopausal women with mean age in years 35.11 ± 4.09 and 62.65 ± 7.4 respectively. Total calcium (mg / ml) and osteocalcin (ng / ml) were evaluated in the serum while hydroxyproline (mg / g creatinine) was estimated in the urine of both the groups. Significant decrease in the total calcium (p value 0.001) and significant increase in the serum osteocalcin level (p value 0.001) and urinary hydroxyproline (p value 0.001) was observed in the postmenopausal women. The results of the study suggested that biochemical markers like osteocalcin and urinary hydroxyproline may serve as the indicators of altered bone metabolism and therefore can be used to monitor the same in postmenopausal women subsequently helping in evaluating the risk of developing osteoporosis.

References

  1. Uebelhart D., Schlemmer A., Johansen J.S., Gineyts E., Christiansen C. and Delmas P.D., Effect of menopause and hormone replacement therapy on the urinary excretion of pyridinium cross links, J Clin Endocrinol Metab., (72), 367-373 (1991)
  2. Manolagas S.C. and Jilka R.L., Bone marrow, cytokines and bone remodeling: emerging insights into the pathophysiology of osteoporosis, N England J Med., (332), 305-311 (1995)
  3. Riggs B.L. and Melton L.J., Medical progress series: involution osteoporosis, N Engl J Med.,(314), 1676-1686, (1986)
  4. Susan A., Calcium Supplementation in postmenopausal women, From medscape Ob/Gyn & Women’s health, http://www.medscape.com/viewarticle/460438, 8(2), (2003)
  5. Carr B.R. and Bradshaw K.D., Disorders of the ovary and female reproductive tract, In: Braunwald e, Isselbacher K.J., Wilson J.D., Martin J.B., Fauci A.S. and Kasper D.L., editors, Harrison’s Principals of Internal Medicine, 14th Ed. New York: Mc Graw Hill, 2097-115, (1998)
  6. Delmas PD, Biochemical markers of bone turnover for the clinical investigation of osteoporosis, Osteoporos Int., 3(1), 81–6, (1993)
  7. Connerty HV, Briggs AR, Determination of serum calcium by means of orthocresolphthalein complexone, Am. J Clin Path.,(45), 290-6, (1966)
  8. Brown JP, Delmas PD, Serum BGP: a specific marker for bone formation in postmenopausal osteoporosis, The Lancet, 1091-1093, (1984)
  9. Bergman I, Loxley R, The determination of hydroxyproline in urine hydrolysates, Clin Chim Acta, (27), 347–9, (1970)
  10. Newman DJ, Price C, Renal function and nitrogen metabolites. In: Brutis CA, Ashwood ER, editors, Tietz Textbook of Clinical Chemistry, 3rd ed. Philadelphia: W.B. Saunders, 1204-70, (1998)
  11. Indumati V, Patil VS, Jailkhani R, Hospital based preliminary study of osteoporosis in postmenopausal women, Ind J Clin Biochem.., 22(2), 96–100, (2007)
  12. Demers LM, Costa L, Lipton A, Biochemical markers and skeletal metastases, Cancer, (88), 2919-2926, (2000)
  13. George BO, Urinary and Anthropometrical Indices of Bone Density in healthy Nigerians adults, J Appl Sci Environ Mgt., 7(1), 19-23, (2003)
  14. Sachdeva A, Seth S, Khosla AH, Sachdeva S, Study of some common Biochemical bone turnover markers in postmenopausal women, Ind J Clin Biochem. , 20(1), 131-4, (2005)
  15. Vanita R. Jagtap, Jayshree V. Ganu. Effect of antiresorptive therapy on urinary hydroxyproline in postmenopausal osteoporosis, Ind J Clin Biochem., 27(1), 90-93, (2012)
  16. Delmas PD. Biochemical markers of bone turnover, J Bone Miner. Res. Int., 8 (Suppl 2), S549-S555, (1993)
  17. Vanita R. Jagtap, Jayashri V. Ganu, Nitin S. Nagane, BMD and Serum Intact Osteocalcin in Postmenopausal steoporosis Women, Ind J Clin Biochem., 26(1), 70-83, (2011)
  18. Civitelli R, Armamento-Villareal R, Napoli N, Bone turnover markers: understanding their value in clinical trials and clinical practice, Osteoporosis Int, (20), 853-51, (2009)
  19. Lee AJ, Hodges S, Eastell R, Measurement of osteocalcin,Ann Clin Biochem, (37), 432-46, (2000)
  20. Ravn P, Fledelius C, Rosenquist C, Overgaard K, Christiansen C, High bone turnover is associated with low bone mass in both pre and postmenopausal women, Bone, (19), 291-8 , (1996)
  21. Filip RS, Zagorski J, Age and BMD related differences in biochemical markers of bone metabolism in rural and urban women from Lublin region, Poland, Ann Agric Environ Med, (11), 255-9, (2004)
  22. Iki M, Akiba T, Matsumoto T, Nishino H, Kagamimori S, Kagama Y, et al. and JPOS Study Group, Reference database of biochemical markers of bone turnover for the Japanese female population, Osteoporos Int., (15), 981–91, (2004)
  23. Pino JD, Gomez EM, Rodriguez MM, Sosa CL, Cordero M, Lanchares JL, Talavera JRG, Influence of sex, age and menopause in serum osteocalcin (BGP) levels, J Mol Med., 69(24),1135–8, (1991)
  24. Osteoporosis in postmenopausal women: Diagnosis and Monitoring. Summary, Evidence Report/ Technology Assessment: No.28, Agency for Healthcare Research and Quality Publication No. 01-E031, http://www.ahrq.gov/ clinic/epcsums/osteosum.htm, (2001)