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Assessment of Bone Loss in Postmenopausal Women by Evaluation of Urinary Hydroxyproline and Serum Status of Osteocalcin

Author Affiliations

  • 1Department of Biochemistry, College of Dental Science and Hospital, Rau, INDIA
  • 2 Department of Biochemistry, Govt. Holkar Science College, Indore, INDIA
  • 3 Department of Biochemistry, MGM Medical College, Indore, INDIA
  • 4

Int. Res. J. Biological Sci., Volume 2, Issue (9), Pages 11-14, September,10 (2013)


Bones are in a dynamic state throughout the life. Bone metabolism is a continuous process which maintains a balance between the resorption of old and injured bone and the formation of new bone under. This continuous degradation and formation of bone is termed as bone remodeling or bone turnover, a process that occurs throughout the life. The balance between bone degradation and bone formation is altered due to aging and cessation of the menstrual cycle in women. The present study aimed at estimation of the two important markers of bone turnover, serum osteocalcin and urinary hydroxyproline in postmenopausal women and also to probe into the role of these both in assessing the altered bone metabolism. 100 women were included in the study out of which 50 were premenopausal who served as the control and 50 were postmenopausal women with mean age in years 35.11 ± 4.09 and 62.65 ± 7.4 respectively. Total calcium (mg / ml) and osteocalcin (ng / ml) were evaluated in the serum while hydroxyproline (mg / g creatinine) was estimated in the urine of both the groups. Significant decrease in the total calcium (p value 0.001) and significant increase in the serum osteocalcin level (p value 0.001) and urinary hydroxyproline (p value 0.001) was observed in the postmenopausal women. The results of the study suggested that biochemical markers like osteocalcin and urinary hydroxyproline may serve as the indicators of altered bone metabolism and therefore can be used to monitor the same in postmenopausal women subsequently helping in evaluating the risk of developing osteoporosis.


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