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Rapid Immunodiagnosis of Tuberculosis by using Polymerase Chain Reaction and in-house developed Enzyme linked Immunosorbent Assay

Author Affiliations

  • 1 Department of Microbiology, Karpagam University, Coimbatore - 641021, Tamil Nadu, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (8), Pages 42-47, August,10 (2013)

Abstract

Tuberculosis (TB) remains the second leading cause of death among all infected individuals and is responsible for 2 million deaths annually. The definitive diagnosis of TB is by demonstrating acid-fast bacilli either by direct smear or culture. Since these methods are insensitive (smear technique) and time consuming (culture technique), additional immunodiagnostic methods such as antigen or antibody demonstration has become necessary. The main purpose of this study is to develop a diagnostic tool which can yield results in short span of time with much higher sensitivity rate. In this study, 200 serum samples were used for the development of ELISA technique. Out of 200 samples, 149 gave positive results and the sensitivity of the developed test appeared to be 88.7%.

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