6th International Virtual Congress (IVC-2019) And Workshop.  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Inoculum Production of Acaulospora laevis using Fresh and decomposed Apple Pomace as Substrate

Author Affiliations

  • 1DAV College for Girls, Yamunanagar, Haryana, INDIA
  • 2 Mycology and Plant Pathology Laboratory, Department of Botany, Kurukshetra University Kurukshetra- 136119, Haryana, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (8), Pages 32-36, August,10 (2013)

Abstract

Because of the biotrophic nature of arbuscular mycorrhizal fungi, it is not been able to use on a commercial scale despite being aware of the potentiality of arbuscular mycorrhizal fungi in agriculture, forestry and horticulture research. For the commercial development of AM inoculants, a number of strategies have been followed time to time with their own merits and demerits. Three plant species viz. wheat, lemon grass and lily grass were examined for mass production of consortium of A. laevis, AM fungus present in the rhizosphere soil after adding different concentration of fresh and decomposed apple pomace as substrate. Out of the three test species, lemon-grass responded as the most suitable host showing highest colonization (89.70.50%; 75.01.58 spores with fresh and decomposed 86.61.90%; 72.21.92 spores substrate. It was also observed that plants having higher AM colonization showed higher AM spore production showing a positive correlation. They not only stimulated AM development, but also accelerated the root and shoot growth.

References

  1. Tiwari P., Prakash A. and Adholya A., Commercialization of arbuscular. mycorhizzal-biofertilizer, Handbook of Fungal Biotechnology, 2nd edition, (Ed.) Arora, D.K., Marcel Decker, Inc. NY, 195-203 (2003)
  2. Ezawa T., Yamamoto K. and Yoshida S., Species composition and spore density of indigenous vesicular- arbuscular mycorrhizal fungi under differentconditions of P- fertility as revealed by soybean trap culture, Soil Sci. Plant Nutr., 46, 291-297 (2000)
  3. Fortin J.A., Becard G., Declerck S., Dalpe Y., St- Arnaud M., Coughlan A.P. and Piche Y., Arbuscular mycorrhiza on root- organ cultures, Can. J. Bot.,80, 1-20 (2002)
  4. Dalpe Y. and Monreal M., Arbuscular mycorrhizal inoculum to support sustainable cropping systems [online], Crop manage, Available from www.plantmanagementnetwork.org /pub/cm /review/2004/amfungi (2004)
  5. Gianinazzi S. and Vosatka M., Inoculum of arbuscular mycorrhizal fungi for the production systems: science meets business, Can. J. Bot.,82, 1264-1271 (2004)
  6. Gerdemann J.W., and Nicolson Y.H., Spores of mycorrhizae Endogone species extracted from soil by wet seiving and decanting, Trans. Brit. Mycol. Soc., 46, 235-244 (1963)
  7. Menge J.A. and Timmer L.M., Procedure for inoculation of plants with VAM in the laboratory, greenhouse and field, 59-68, In: Schenck, N.C. (ed.), Methods and Principles of Mycorrhizal Research. A.P.S. Press, St. Paul, Minnesota (1982)
  8. Phillips J.M. and Hayman D.S., Improved procedures for clearing roots and staining parasitic and VAM fungi for rapid assessment of infection, Trans. Brit. Mycol. Soc.,55, 158-161 (1970)
  9. Walker C., Taxonomic concepts in the Endogonaceae.II: A fifth morphological wall type in endogonaceous spores, Mycotaxon,25, 95-105 (1986)
  10. Mortan J.B. and Benny G.L., Revised classification of arbuscular mycorrhizal fungi (Zygomycetes), New order Glomales, two new sub orders Glomineae and Gigasporineae and two new families Acaulosporaceae and Gigasporaceae with emendation of Glomaceae, Mycotaxon, 37, 471-491 (1990)
  11. Adholeya A. and Gaur A., Estimation of VAM fungal spores in soil, Myco.News,6(1), 10-11 (1994)
  12. Baby U.I. and Manibhushanrao K., Influence of organic amendments on arbuscular mycorrhizal fungi in relation to rice sheath blight disease, Mycorrhiza, 6, 201-206 (1996)
  13. Muthukumar T. and Udaiyan K., Gigaspora decipiens(Glomales) an arbuscular mycorrhizal fungus from western ghats of Southern India, J.Mycol. Pl. Pathol., 32(1), 96-99 (2002)
  14. Gryndler M., Jansa J., Hrselova H., Chvatalova I. and Vosatka M., Chitin stimulates development and sporulation of arbuscular mycorrhizal fungi, Appl.Soil Ecol., 22, 283-287 (2003)
  15. Jeffries P. and Barea J.M., Arbuscular mycorrhiza: a key component of sustainable plant- soil ecosystems, In: The Mycota: fungal associations (ED.) Hock, B., Vol. IX. Berlin. Herdelberg, New York: Springer, 95-113 (2001)
  16. Cavender N.C.D., Atiyeh R.M. and Knee M., Vermicompost stimulates myco- colonization of roots of Sorghum bicolor at the expense of plant growth, Pedobiologia,47(1), 85-89 (2003)
  17. Tanwar A., Kumar A., Mangla C. and Aggarwal A., Mass multiplication of G. mosseae using different hosts and substrate, J. Mycol. Pl.Pathol.,40(2), 306-308 (2010)
  18. Mahmood I. and Rizvi R., Mycorrhiza and organic farming, Asian J. Plant Sci.,, 241-248 (2010)
  19. Chaturvedi S., Mehta C.M., Singh S. and Sharma A.K., Host influences arbuscular mycorrhizal fungal diversity, J. Mycol. Pl. Pathol,39(1), 124-130 (2009)
  20. Giovannetti M., Sbrana C., Aviol, Citernesi, AS and Logi, C., Differential hyphal morphogenesis in arbuscular mycorrhizal fungi during preinfection stages, New Phytol., 125, 587-593 (1993)
  21. Scheloske S., Maetz M., Schneider T., Hildebrandt U., Bthe H. and Povh B., Element distribution in mycorrhizal and non- myco- roots of the halophyte Aster tripolium determined by proton induced x- rays emission, Protoplasma, 223, 183-189 (2004)
  22. Sharma, S, Aggarwal, A, Parkash, V and Sharma, D., Mass production of VAM fungi using different substrate and hosts, J. Mycopathol. Res., 43(1): 51-56 (2005)
  23. Chauhan S., Kumar A., Mangla C. and Aggarwal A., Inoculum production of endomycorrhizal fungi: effect of hosts and substrates in rapid culturing of Glomus mosseae. Cont. J. Biol. Sci., 4(2), 6-12 (2011)