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Identification of Rice Kinesin 13A Interacting Proteins through Yeast Two-Hybrid Screening

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Author Affiliations

  • 1 Department of Integrative Plant Science, Chung-Ang University, Anseong 456-756, KOREA
  • 2 National Academy of Agricultural Science, RDA, Suwon 441-707, KOREA

Int. Res. J. Biological Sci., Volume 2, Issue (8), Pages 20-26, August,10 (2013)

Abstract

By using full-length rice kinesin 13A protein (OsKIN13A) as a bait construct and rice cDNA library as a prey, we obtained several interacting proteins through yeast two-hybrid (Y2H) screening. These interacting proteins were selected by beta-galactose filter assay after growing on SD-TLH media containing 25 mM of 3-aminotriazole (3-AT). All of selected interacting proteins were confirmed by sequencing and annotated according to the rice genome database, and grouped into seven functional categories, i.e. cellular processes, transport and binding protein, metabolic activity, signal transduction, molecular chaperone and other category. This study suggests that OsKIN13A protein is associated with wide range of biological processes such as development, signaling and immunity.

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