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Comparison of Sweet taste Suppression by Callus with different Leaf Explants of Gymnema sylvestre

Author Affiliations

  • 1 Tanveer Malawat College of Biosciences, Bikaner, Rajasthan, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (8), Pages 73-75, August,10 (2013)

Abstract

To explore the possibility of in vitro production of gymnemic acid in cell & callus cultures at commercial level, callus was regenerated from leaf and nodal explants of Gymnema on MS medium supplemented with different combinations of auxins and cytokinins. Best response (100%) was observed with leaves incubated on MS + 0.5mgl-1Kn+ 1.5 mgl-1 2,4-D.For estimating GA content, recovery time of sweet taste after chewing the sample was taken as parameter. Callii obtained from leaves from juvenile and mature plates were compared for GA activity. Samples were chewed separately by volunteers of different age groups (10-40years) before and after taking meal. Maximum time (4 hours) in sweet taste recovery was observed by 10-20 years old volunteers by chewing mature leaves before taking meal, while with callii this time was very short (15-20min).Callus obtained on different PGR combinations responded differently. Hence by altering media composition production of gymnemic acid can be enhanced and such oral test can be used as preliminary test for estimation of GA in sample.

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