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Molecular Characterization of Pleurotus ostreatus PO-3 involved in Mycoremediation of Benzo[a]Pyrene

Author Affiliations

  • 1Dept. of Microbiology, Karpagam University, Coimbatore-641021, Tamil Nadu, INDIA
  • 2 Dept. of Microbiology, Genohelix Biolabs, CASB, Jain University, Bangalore-560019, Karnataka, INDIA
  • 3 Dept. of Microbial Biotechnology, Bharathiar University, Coimbatore-641046, Tamil Nadu, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (8), Pages 48-53, August,10 (2013)

Abstract

Benzo[a]pyrene is a recalcitrant organic pollutant mainly because of the low water solubility which makes it unavailable for microbial degradation. Besides being ubiquitous in the environment, it is considered as a priority pollutant because of its carcinogenic, teratogenic and mutagenic effects. White-rot fungi such as Pleurotus ostreatus had been the organism of choice for the degradation of benzo[a]pyrene. The present study aims at the molecular identification of the selected fungal isolate by partial 18S rDNA sequencing and comparative evaluation of the benzo[a]pyrene biodegradation potential of both wild isolate and reference strain of Pleurotus ostreatus MTCC 142.Results indicated that the fungal isolate PO-3 was closely related to Pleurotus ostreatus strain Po-13 with 99% sequence similarity. The 703 bp 18S rDNA nucleotide sequence was provided a GenBank accession number KC986398. In terms of degradation potential, PO-3 isolate was a better degrader of 1g/ml of benzo[a]pyrene. Level of degradation was 32% and 29% respectively for Pleurotus ostreatus isolate PO-3 and Pleurotus ostreatus MTCC 142. Thus based on the present finding, Pleurotus ostreatus PO-3 may prove to be a promising isolate for degradation of benzo[a]pyrene.

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