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A study over pattern of Zinc Tolerance among Rhizobial isolates of Trifolium alexandrinum

Author Affiliations

  • 1Department of Microbiology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Manduwala, Dehradun, Uttarakhand, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (7), Pages 12-18, July,10 (2013)


All over the world Heavy metal pollution of soil is a severe environmental problem and has drastically increased due to beginning of industrillization. This pollution affects the growth ,morphology and metabolic activities of soil microorganisms through functional disturbance of enzyme activities and protein denaturation. Organisms which can resist such conditions provides an economical and cheap alternative technology to clean up heavy metal contaminated soils. Characterisation, CFU/ml counts , biomass yield., enzymes (cellulase and amylase) and proteins of zinc tolerant and sensitive Rhizobium sp. In the present study bacteria were isolated from the Barseem (Trifolium alexandrinum) root nodules. On the basis of colonial, morphological, biochemical and nodulation ability it was found that all the isolates belongs to Rhizobium species. On the basis of CFU/ml counts and biomass yield zinc sensitive isolates were selected Based on the above parameters, it was concluded that the strain R1 is most sensitive and the strain R2 is tolerant to zinc. The tolerant strain produces an increased amount of the enzymes (cellulase and amylase) and proteins than the sensitive strain. The similarity coefficient calculated revealed that the tolerant and the commercial (MTCC 905) strains are 72.3% similar. The R2 strain had the highestlevel of enzymes (cellulase and amylase) and proteins.


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