9th International Science Congress (ISC-2019).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

A study over pattern of Zinc Tolerance among Rhizobial isolates of Trifolium alexandrinum

Author Affiliations

  • 1Department of Microbiology, Dolphin (PG) Institute of Biomedical and Natural Sciences, Manduwala, Dehradun, Uttarakhand, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (7), Pages 12-18, July,10 (2013)

Abstract

All over the world Heavy metal pollution of soil is a severe environmental problem and has drastically increased due to beginning of industrillization. This pollution affects the growth ,morphology and metabolic activities of soil microorganisms through functional disturbance of enzyme activities and protein denaturation. Organisms which can resist such conditions provides an economical and cheap alternative technology to clean up heavy metal contaminated soils. Characterisation, CFU/ml counts , biomass yield., enzymes (cellulase and amylase) and proteins of zinc tolerant and sensitive Rhizobium sp. In the present study bacteria were isolated from the Barseem (Trifolium alexandrinum) root nodules. On the basis of colonial, morphological, biochemical and nodulation ability it was found that all the isolates belongs to Rhizobium species. On the basis of CFU/ml counts and biomass yield zinc sensitive isolates were selected Based on the above parameters, it was concluded that the strain R1 is most sensitive and the strain R2 is tolerant to zinc. The tolerant strain produces an increased amount of the enzymes (cellulase and amylase) and proteins than the sensitive strain. The similarity coefficient calculated revealed that the tolerant and the commercial (MTCC 905) strains are 72.3% similar. The R2 strain had the highestlevel of enzymes (cellulase and amylase) and proteins.

References

  1. Adriano, D.C., Trace elements in terrestrial environments; Biochemistry, bioavailability and risks of metals. SpringerVerlag, New York.2nd Edition,866 (2001)
  2. Gianfreda L., Rao M.A., Piotrowska A., Palumbo G. and Colombo C., Soil enzymes activities as affected by anthropogenic alterations: intensive agricultural practices and organic pollution, Science of Total Environ, 341, 256-279 (2005)
  3. Dick W.A. and Tabatabai M.A., Potential uses of soil enzymes. In: metting B editor. Soil Microbial ecology, New York : Marcel Dekke: 95-127 (1992)
  4. Bandick A.K. and Dick R.P., Field management effects on soil enzyme activities, Soil Biol and Biochem, 31, 1471-1479 (1999)
  5. Heaton A.C.P., Rugh C.L., Wang N.J. and Meagher R.B., Phytoremediation of mercury- and methylmercury-polluted soils using genetically engineered plants, J Soil Contamination, 7, 497-509 (1998)
  6. Chaudri A.M., Lawlor K., Preston S., Paton G.I., Killham K. and McGrath S.P., Response of a Rhizobium-based biosensor to Zn and Cu in soil solutions from sewage sludge treated soils, Soil Biol and Biochem, 32, 383-388 (2000)
  7. Smith S.R., Rhizobium in soils contaminated with copper and zinc following the long-term application of sewage sludge and other organic wastes, Soil Biology and Biochemistry, 29, 1475-1489 (1997)
  8. Nies D.H., The cobalt, zinc, and cadmium efflux system CzcABC from Alcaligenes eutrophus functions as a cationproton antiporter in Escherichia coli. J Bac, 177, 2707-12 (1995)
  9. Singh B., Kaur R. and Singh K., Characterization of Rhizobium strain isolated from Trigonella foenumgraecum (Fenugreek), African J. Biotech., 7(20), 3671-3676 (2008)
  10. Holt J.G., Krieg N.R., Sneath P.H.A., Staley J.T. and Williams S.T., In Bergey’s Manual of Determinative Bacteriology, Williams and Wilkins Press, Baltimore, USA (1994)
  11. Vincent J.M., A manual for the practical study of rootnodule bacteria. Blackwell Scientific Publications, Oxford (1970)
  12. Miller G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem., 31, 426 (1959)
  13. Lowry D.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein determination of the Lowry Assay, J. Biol. Chem., 193, 265-275 (1951)
  14. Lupwayi N. and Haque I., Legume-Rhizobium Technology Manual. Environmental Sciences Division International Livestock Centre for Africa, Addis Ababa, Ethiopia, 1-93(1994)
  15. Wei G.H., Tan, E.T., Zhu, M.E., Wang. E.T., Han, S.Z. and Chen W.X., Characterization of rhizobia isolated from legume species within the genera Astragalus and Lespedeza grown in the Loess Plateau of China and description of Rhizobium loessense sp. Int. J. Evol. Microbiol., 53, 1575- 1583 (2003)
  16. Hunter W.J., Kuykendall L.D. and Manter D.K., Rhizobium selenireducens sp. nov.: A Selenite-Reducing - Proteobacteria Isolated From a Bioreactor, Curr. Microbiol., 55, 455-460 (2007)
  17. De Oliveira A.N., de Oliveira L.A., Andrade J.S. and Chagas JAF., Rhizobia amylase production using various starchy substances as carbon substrates, Braz. J. Microbiol., 38, 208-216 (2007)
  18. Hajnaa A., Triple-Sugar Iron Medium for the identification of the intestinal group of bacteria, J. Bacteriol., 49, 516-517(1945)
  19. Bogic M., Dusica D., Olivera S., Natasa R., Dorde K. and Dragana J., Effects of heavy metals on rhizobial growth,Roumanian Biotechnology Letters, 11(6), 2995-3003 (2006)
  20. Gauri, Singh A.K., Bhatt R.P., Pant S. and Bedi M., Effect of Zinc on nodulation and growth parameters of Trifolium alexandrinum, Crop Res. 40(1,2&3), 186-191 (2010)
  21. Angle, J. and Chaney R., Heavy metal effects on soil populations and heavy metal tolerance of Rhizobium meliloti, nodulation and growth of alfalfa, Water, Air and Soil Pollution, 57, 597-604 (1991)
  22. Arlem Nascimento de Oliveira, Luiz Antonio de Oliviria and Jerusa Souza Andrade., Partial characterization of amylases of two indigenous Central Amazonian rhizobia strains, Braz. Arch. Boil. Technol. 53(1), 35-45 (2010)
  23. Sofia Isabel Almeida Pereira, Ana Isabel Gusmão Lima and Etelvina Maria de Almeida Paula Figueira, Heavy metal toxicity in Rhizobium leguminosarum biovar viciae Isolated from soils subjected to different sources of heavymetal contamination: Effects on protein expression, App.Soil .Ecol., 33, 286-293 (2006)