International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

A lignolytic Fungi with Laccase Activity Isolated from Malaysian local Environment for Phytochemical Transformation Purposes

    , ,

Author Affiliations

  • 1 Dept. of Biotechnology, Kulleyyah (Faculty) of Science, International Islamic University Malaysia, Kuantan, Pahang, MALAYSIA

Int. Res. J. Biological Sci., Volume 2, Issue (2), Pages 51-54, February,10 (2013)

Abstract

The main aim of the current study is to isolate a fungi with laccase activities to be used for transformation of phytochemicals isolated from the same environment as an approach to produce novel pharmaceutical products. In our study, laccase producing fungi was isolated from samples taken from a tropical trees barks which grown in the International Islamic University Malaysia, Kuantan campus using different chromogenic substrates on the agar plates such as ABTS, Tannic Acid, Azure B and Guaiacol. It was found the isolated fungi has excellent ability to produce laccase. The conclusion of this study confirm here that using ABTS is very useful to isolate laccase producing fungus. The microscopic visualization showed the characteristics of white-rot fungi. Further molecular identification still ongoing to check the complete identity of our fungi.

References

  1. Desai S.S., Tennali B.G., Channur N., Anup A.C., Deshpande G., Azhar Murtuza B.P., Isolation of laccase producing fungi and partial characterization of laccase, Biotechnol. Bioinf. Bioeng., 1,543-549 (2011)
  2. Heinzkill M., Bech L., Halkier T., Schneider, P., Anke T., Characterization of laccase and peroxidase fromwood-rotting fungi (family Coprinaceae), Appl. Environ. Microbiol., 64, 1601-1606 (1998)
  3. Garzillo A.M.V., Colao M.C., Caruso C., Caporale C., Celletti D., Buonocore V., Laccase fromthe white-rot fungus Trametes trogii. Microbiol. Biotechnol., 49, 545-551 (1998)
  4. priyadarsinin I.R., Bhuraneswari V., Kumar S.K., ISOLATION, Identification and phylogenetic analysisa of white rot fungus and heterologus expression of gene encoding laccase, Journal of Applied Sciences in Environmental Sanitation, 6, 69-83 (2011)
  5. Givaudan A., Effosse A., Faure D., Potier P., Bouillant M.L., Bally R., Polyphenol oxidase in Azospirillum lipoferum isolated from rice rhizosphere: evidence for a laccase in non-motile strains of Azospirillum lipoferum, FEMS Microbiol Lett., 108, 205–210 (2004)
  6. Kiiskinen L.L., Ratto M., Kruus K. J., Screening for novel laccase-producing microbes, Journal of Applied Microbiology, 97, 640-646 (2004)
  7. Poojary H. and Mugeraya G., Laccase Production by Phellinus noxius hpF17: Optimization of Submerged Culture Conditions by Response Surface Methodology, Research in Biotechnology, 3,09-20 (2012)
  8. Dashtban M., Schraft H., Syed T.A., Qin W., Fungal biodegradation and enzymatic modification of lignin, Int. J. Biochem. Mol. Biol., 1, 36–50 (2010)
  9. Caparros-Ruiz D., Fornale S., Civardi L., Puigdomenech P., Rigau J., Isolation and characterization of a family of laccases in maize, Plant Sci., 171, 217-225 (2006)
  10. Maciel M.J.M., Silva A.C., Ribeiro H.C.T., Industrial and biotechnological applications of lignolytic enzymes of the basidiomycota: a review, Electron. J. Biotechnol., 13, 1-12 (2010)
  11. Pointing S. B., Qualitative methods for the determination of lignolcellulotytic enzyme production by tropical fungi, Fungal Diversity,2, 17-33 (1999)
  12. Kumar V.V., Kirupha D.S., Periyaraman P., Sivanesan S., Screening and induction of laccase activity in fungal species and its application in dye decolorization, African Journal of Microbiology Research, 5, 1261-1267 (2011)
  13. Sivakumar R., Rajendran R., Balakumar C., Tamilvenan M., Isolation, screening and optimization of production medium for thermostable laccase production from Ganoderma sp., International Journal of Engineering Science and Technology, 2,7133-7141 (2010)
  14. Viswanath B., Chandara S. M., Pallavi H., Reddy R. B., Screening and assessment of laccase producing fungi isolated from different environmental samples, African Journal of Biotechnology, 7, 1129-1133 (2008)
  15. Machado K.M.G., Matheus D.R., Bononi V.L.R., Ligninolytic enzymes production and Remazol Brilliant Blue R decolorization by tropicalBrazilian basidiomycetes fungi. Brazilian J. Microbiol., 36, 246-252 (2005)