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Comparative study of Glycerate Kinase (GK): Bioinformatical Approach

Author Affiliations

  • 1 Department of Biotechnology, The University of Burdwan, Golapbag, Burdwan, 713104, West Bengal, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (12), Pages 50-59, December,10 (2013)


There are three classes of Glycerate kinase (GK) which are class I GK, class II GK and class III GK. Class I and class II GKs produce glycerate 2-phosphate whereas class III GK (GLYK) only can produce glycerate 3-phosphate. Phylogenetic analysis on 16S ribosomal RNA sequences reveals the strong evolutionary relationship between cyanobacteria and plants. Phylogeny using GK DNA and amino acid sequences shows that cyanobacteria group is closely related with both bacteria and plants whereas fungi are closely related only with plants. Phylogeny using the amino acid sequence and hierarchical clustering on the basis of the amino acid frequencies of GK shows similar relationship among the taxa. Hierarchical clustering on the basis of GC% of GK encoding gene showing the unusual property like the RSCU value of the codons UUG and AGG are significantly low and CGA is significantly high in GC rich cluster. Correlation coefficient between GC% and the amino acids arginine, tryptophan and serine shows that the plants are different from the other selected species. ENc plot shows that except few GK genes from fungi and gammaproteobacteria all of them are under mutational bias. There is no as such codon usage similarity for the GK encoding gene from different organisms but they have similar degree of expression i.e, CAI (highest in plant) which is significantly low along with the amino acids lysine, phenylalanine, tyrosine, isoleusine and asparagine and serine in GC rich GK encoding gene.


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