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Primary screening, Characterization and Seasonal variations of Aspergillus and Penicillium species in the Black cotton soils (Vertisols) of Salur Mandal, Viziyanagaram District, Andhra Pradesh, India

Author Affiliations

  • 1 Centre of Advanced Study, Department of Botany, Andhra University, Visakhapatnam-530003, AP, INDIA
  • 2 Department of Botany, Centre for Research, Dr. V.S. Krishna Govt. College, Visakhapatnam-530013, AP, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (12), Pages 30-41, December,10 (2013)


Fungi are an important component of the soil microbiota typically constituting more of the soil biomass than bacteria, depending on soil depth and nutrient conditions. This study deals with the primary screening, characterization and seasonal variations of mycoflora, isolated from twelve soil samples. The soil samples were collected from agricultural fields of five different locations at Salur Mandal during three seasons such as Kharif, Rabi and summer respectively. Dilution plate technique was used to isolate soil fungi from various soil samples. Fungal isolates were screened on different culture media namely Potato Dextrose Agar, Czapeks Dox Agar, and Sabouraud’s Dextrose Agar supplemented with 1% Streptomycin. Fungal colonies were counted and screened for the occurrence of different fungal species along with Aspergillus and Penicillium presented in soil samples. Identification of the soil isolates were made with help of the relevant literature and standard manuals of soil fungi. Mycoflora including Aspergillus (12 species), Penicillium (6species), Trichoderma (3 species), Cunninghamella (2 species), Curvularia (2 species), Fusarium (2 species), Mucor (2 species) and Rhizopus (1 species) were identified with the help of taxonomic keys. Among the isolates the genera Aspergillus and Penicillium were dominant due to their sporulation ability. The periodicity of occurrence of each species was calculated by using data analysis.


  1. Olson R.K., Schoeneberger M.M. and Aschmann S.G., An Ecological Foundation for Temperate Agroforestry. In: North America Agroforestry: An Integrated Science and Practice. Garrett H.E.,W.J.Rietveld and R.F.Fisher (Eds.), American society of Agronomy, Madison, Wisconsin, USA, 31-61 (2000)
  2. Parrotta J.A., Productivity, nutrient cycling and succession in single- and mixed-species plantations of Casuarina equisetifolia,Eucalyptus robusta and Leucaena leucocephala in Puerto Rico, For. Ecol. Manage 124),45-77 (1999)
  3. Sall S.N., Masse D., Reversat F.B., Guisse A. and Chotte J.L., Microbial activities during the early stage of laboratory decomposition of tropical leaf litters: the effect of interactions between the litter quality and exogenous inorganic nitrogen, Biol. Fert. Soils, 39), 103-111 (2003)
  4. Domsch K.H., Gams W. and Anderson T.H., Compendium of soil fungi, London, Academic press, (1980 )
  5. Pitt J.I., The Genus Penicillium and Teleomorphic States Eupenicillium and Talaro-myces, London, Academic Press, Inc. (1979)
  6. Christensen M., Frisvad J.C., Tuthill D.E., Penicillium species diversity in soil and Taxonomic and ecological notes ,Harwood Academy Publishers, 309-320 (2000)
  7. Klich M.A., Identification of Common AspergillusSpecies, Utrecht, The Netherlands:Centraalbureau voor Schimmelcultures, 122 (2002)
  8. Asan A., Aspergillus, Penicillium and related species from Turkey, Mycotaxon, 89, 155-157 (2004)
  9. Carroll G.C. and Wicklow D.T., The Fungal Community: Its Organization and Role in the Ecosystem, New York, Marcel Dekker, Inc. (1992)
  10. Christensen M., Species diversity and dominance in fungal communities, In:Wicklow DT, Carroll GC (eds), The Fungal Community: Its Organization and Role in the Ecosystem,Marcel Dekker, Inc. New York, 201-232 (1981)
  11. Lodge D.J., Factors related to diversity of decomposer fungi in tropical forests, Biodivers. Conserv., 681-688 (1997)
  12. Amakiri M.A., Microbial Degradation of soil applied herbicides, Nig. J.Microl., 17-21 (1982)
  13. Kolhe, A. H., Chandran P., Ray S. K., Bhattacharyya T., Pal D. K. and Sarkar D., Genesis of associated red and black shrink– swell soils of Maharashtra, Clay Res, 30, 1–11 (2011)
  14. Dudal R., Dark Clay Soils of Tropical and Subtropical Regions, Agric. Dev., FAO, Rome, Italy, 83,161 (1965)
  15. Warcup J.H., On the origin of colonies of fungi developing on soil dilution plates, Trans. Brit. Mycol .Soc, 38, 298–301, (1955)
  16. Ali S,. Ikram-ul-Haq., Qadeer M.A., Iqbal J., Production of citric acid by Aspergillus niger using cane molasses in a stirred fermentation, E. J. Biotech, (), 1 (2002)
  17. Onions A.H.S., Allsopp D., Eggins H.O.W., Smith’s Introduction to Industrial Mycology, 7th Edition, Edward Arnold, London, 372 (1981)
  18. Okunowo W.O. and Ogunkanmi L.A., Effects of sodium ion and water hyacinth extract in the production of Curvularia pallescens in culture media, Afr. J. Biochem. Res.,3(5), 238-244 (2009)
  19. Zhang WM., Moody K. and Watson AK., Responses of Echinochloa species and rice (Oryza sativa) to indigenous pathogenic fungi, Plant Dis, 80, 1053-1058 (1996)
  20. Gilman J.C., A Manual of Soil fungi, 2nd Indian edition, Biotech Books, Delhi (2001)
  21. Nagamani A., Kunwar I.K. and Manoharachary C., Hand book of soil fungi, I.K.International Pvt.Ltd (2006)
  22. Waksman S.A., Three decades with soil fungi, Soil Sci.58, 89-114 (1944)
  23. Marschner P., Kandeler E. and Marschner B., Structure and function of the soil microbial community in a long- term fertilizer experiment , Soil Biol. Biochem, 35, 453-461 (2003)
  24. Mohanty R.B. and Panda T., Ecological studies of the soil microfungi in a tropical forest soil of South Orissa in relation to deforestation and cultivation, J. Ind. Bot. Soc, 73, 213-216 (1994b)
  25. Behera N. and Mukherji K.G., Seasonal variation and distribution of micro fungi in forest soils of Delhi, Folia. Geo. Bot. Et. Phyto, 20, 291-312 (1985)
  26. Mamtaz S.D. and Mishra R.R., Decomposition of Maize Zea mays) crop residues, J. Ind. Bot. Soc, 70, 135-138 (1991)
  27. Mohanty R.B. and Panda T., Survey of Penicillous fungi in South Orissa soils, Pl. Sci. Res,16 (), 51-53 (1994a)
  28. Manoharchary C., Sridhar K., Singh R., Adholeya A., Rawat S. and Johri B.N., Fungal biodiversity, distribution, conservation and prospecting of fungi from India, Curr. Sci, 89), 59-70 (2005)
  29. Ayansina A.D.V. and Oso B.A., Effect of commonly used Herbicides on soil microflora at two different concentrations, Afr. J. Biotechnol, ),129-13 (2006)
  30. Yu C., Lv D.G., Qin S.J., Du G.D. and Liu G.C., Microbial flora in Cerasus sachalinensis rhizosphere, Chinese. J. Appl. Ecol , 18 (10), 2277-2281 (2007)
  31. Dong A.R., Lv G.Z., Wu Q.Y., Song R.Q. and Song F.Q ., Diversity of soil fungi in Liangshui natural reserve, Xiaoxing’anling forest region, J. Northeast Forestry University, 32), 8-10 (2004)
  32. Song F.Q., Tian X.J., Li Z.Q., Yang C.L., Chen B., Hao J.J. and Zhu J., Diversity of filamentous fungi in organic layers of two forests in Zijin Mountain, J. Forestry. Res, 15 (), 273-279 (2004)
  33. Zhang C.B., Jin Z.X. and Li J.M., Diversity of bacterial physiological groups and microbial flora in the soil of eight forest types of Tiantai Mountain, Zhejiang, Biodiversity Sic, ), 382-388 (2001)
  34. Jha D.K., Sharma G.D. and Mishra R.R., Ecology of soil micoflora and mycorrhizal symbionts in degraded forests at two altitudes, Biol.Fert.Soils, 12, 272-278 (1992)