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Comparative Study of Extraction Methods for Intracellularly Produced Glucose Isomerase by Streptomyces sp. SB – AII4

Author Affiliations

  • 1Department of Biosciences, Maharaja Ranjit Singh College of Professional Sciences, Khandwa Road, Indore, MP, INDIA
  • 2 Department of Life Sciences, University School of Sciences, Gujarat University, Ahmedabad, Gujarat, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (10), Pages 43-50, October,10 (2013)

Abstract

An array of diverse kinds of Actinomycetes was screened for production of Glucose isomerase. Isolates were analyzed for the production of extracellular and intracellular glucose isomerase. The isolate Streptomyces sp. SB AII4 was identified as the highest intracellular GI yielding strain. The isolate produced GI extracellularly as well as intracellularly. The optimum fermentation period required for GI production was 96 hours at 30\rC and 100 RPM. A comparative analysis was done for maximum extraction of intracellular enzyme. Treatment with CTAB and Ultrasonication were found to be efficient methods for extraction of glucose isomerase.

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