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Effect of Pharmaceutical Effluent on Morphological Parameters and Chlorophyll Content of Cicer arietinum and Vigna radiate

Author Affiliations

  • 1Dept. of Biochemistry, Govt. Holkar Science College, Indore, Madhya Pradesh, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (10), Pages 12-17, October,10 (2013)

Abstract

Experiment was conducted to analyse whether pharmaceutical effluent used to irrigate crops is safe or not. Germinated seedlings of Cicer arietinum (black gram) and Vigna radiata (moong) were treated with varying dilutions of untreated pharmaceutical effluents (100 %, 75%, 50%, 25% and 0%) and their effect was calculated on various parameters like viability %, germination%, vigour index, root length, shoot length, dry weight, fresh weight and chlorophyll. From the results of experiment, it was observed that highest reduction in viability % (6%), germination% (13.89%), vigour index (16.01%), root length (16.95%), shoot length (26.50%), fresh weight (22.33%), total chl (36.36%), chl a (28.57%), chl b (37.57%) was obtained at 0% dilution of pharmaceutical effluent while the highest reduction in dry weight (13.11%) was at 75% of pharmaceutical effluent in Cicer arietinum. In case of Vigna radiata the highest reduction in viability % (5%), germination% (2.78%), vigour index (41.74%), root length (38.26%), shoot length (42.06%), fresh weight (22.33%), dry weight (41.17%), chl a (28.57%), chl b (37.67%) was obtained at 0% dilution of pharmaceutical effluent while in case of total chl (20%) maximum reduction was found at 75%.The results of present study showed that pure pharmaceutical effluent is not safe for irrigation but in case of scarcity of water, pharmaceutical effluent could safely be used for irrigation purpose after proper processing and dilution.

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