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Influence of Growth Media on Hydrphobicity of Phenol-Utilizing Bacteria Found in Petroleum Refinery Effluent

Author Affiliations

  • 1Department of Microbiology, Federal university of Technology, P.M.B.1526, Owerri, NIGERIA
  • 2 Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Port Harcourt, NIGERIA

Int. Res. J. Biological Sci., Volume 2, Issue (10), Pages 6-11, October,10 (2013)

Abstract

The effect of growth medium on hydrophobicity of phenol-utilizing bacteria isolated from petroleum refinery effluent was investigated. The hydrophobicity expression of the isolates were assessed via BATH, SAT and CRB assays. Four different growth media: tryptone soy broth (TSB), nutrient broth (NB), peptone water (PW) and Bushnell Haas broth (BH) was used. All the test isolates exhibited high to moderate hydrophobicity when grown on all the media. However, using SAT assay, Pseudomonas sp. RWW was found to be strongly hydrophobic (1.0) when grown in all the media while Escherichia sp. OPWW was found to be moderately hydrophobic (1.0 – 2.0) in all the media using the same SAT assay. Using Congo red binding assay, Bacillus sp. RBD was found to express weak hydrophobicity when grown in BH medium with Congo red up take value of 9.0 ΅g. All the media used for the assays were observed to be quite well for expression of hydrophobicity by refinery effluent bacteria in the following order: TSB &

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