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Phylogenetic Relationship of Some Ipomoea Seed Proteins by SDS-PAGE

Author Affiliations

  • 1Department of Botany Bangalore University, Bangalore, Karnataka 560056, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (10), Pages 64-67, October,10 (2013)

Abstract

To investigate the phylogenetic relationship of nine Ipomoea species, seed proteins were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Based on the analysis, total 50 bands were identified. The number of bands varies from 8 bands in Ipomoea obscura to 4 in Ipomoea mauritiana. Phylogenetic tree was constructed based on the presence or absence of protein bands using Freetree and Treeview software programme.

References

  1. Saensouk S., The Family Convolvulaceae in Muang District, Nongkhai province Thailand, KKU Res. J., 12(3), 237-243 (2007)
  2. Judd S.W., Campbell S.C., Kellogg A.E., Stevens F.P. and Donoghue J.M., Plant systematics: A Phylogenetic Approach 2nd ed. Massachusettes, USA: Sinauer Associates,Inc. (2002)
  3. Biju S.D., Taxonomic and morphologic studies on the family Convolvulaceae of southern peninsular India., PhD. thesis submitted to Calicut University, Kerala, (1997)
  4. Das S. and Mukherjee K.K., Morphological and biochemical investigations on Ipomoea seedlings and their species interrelationships, Ann. Bot., 79, 565-571 (1997)
  5. Miller R.E., Rausher M.D., Manos P.S., Phylogenetic systematics of Ipomoea (Convolvulaceae) based on ITS and waxy sequences, Syst Bot.,24, 209-227 (1999)
  6. Wilkin P., A morphological cladistic analysis of the Ipomoeae (Convolvulaceae), Kew Bull, 54, 853-876 (1999)
  7. Manos P.S., Miller R.E. and Wilkin P., Phylogenetic analysis of Ipomoea, Argyreia, Stictocardia and Turbina suggests a generalized model of morphological evolution in morning glories, Syst Bot.,26, 585-602 (2001)
  8. Laemmli U.K., Cleavage of structural proteins during assembly of the head of bacteriophage T4, Nature, 227, 680-685 (1970)
  9. Bradford M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding, Anal. Biochem., 72, 248-254 (1976)
  10. Pavlicek A., Hrda S. and Flegr J., Free tree free ware program for construction of phylogenetic trees on the basis of distance data and bootstrap/Jack Knife analysis of the tree robustness, Application in the RAPD analysis of genus Frenkelia, Folia Biologiica., 45, 97-99 (1999)
  11. Jaccard P., Nouvelles rescherches sur la distribution florale, Bull. Soc. Vaud. Sci. Nat., 44, 223-270 (1908)
  12. Roderic D.C., Treeview 32, Versionl. Retrieved from: http:// taxonomy, zoology, gla. ac. uk. /rod/rod. Html., (2001)