Phylogenetic Relationship of Some Ipomoea Seed Proteins by SDS-PAGE
Author Affiliations
- 1Department of Botany Bangalore University, Bangalore, Karnataka 560056, INDIA
Int. Res. J. Biological Sci., Volume 2, Issue (10), Pages 64-67, October,10 (2013)
Abstract
To investigate the phylogenetic relationship of nine Ipomoea species, seed proteins were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Based on the analysis, total 50 bands were identified. The number of bands varies from 8 bands in Ipomoea obscura to 4 in Ipomoea mauritiana. Phylogenetic tree was constructed based on the presence or absence of protein bands using Freetree and Treeview software programme.
References
- Saensouk S., The Family Convolvulaceae in Muang District, Nongkhai province Thailand, KKU Res. J., 12(3), 237-243 (2007)
- Judd S.W., Campbell S.C., Kellogg A.E., Stevens F.P. and Donoghue J.M., Plant systematics: A Phylogenetic Approach 2nd ed. Massachusettes, USA: Sinauer Associates,Inc. (2002)
- Biju S.D., Taxonomic and morphologic studies on the family Convolvulaceae of southern peninsular India., PhD. thesis submitted to Calicut University, Kerala, (1997)
- Das S. and Mukherjee K.K., Morphological and biochemical investigations on Ipomoea seedlings and their species interrelationships, Ann. Bot., 79, 565-571 (1997)
- Miller R.E., Rausher M.D., Manos P.S., Phylogenetic systematics of Ipomoea (Convolvulaceae) based on ITS and waxy sequences, Syst Bot.,24, 209-227 (1999)
- Wilkin P., A morphological cladistic analysis of the Ipomoeae (Convolvulaceae), Kew Bull, 54, 853-876 (1999)
- Manos P.S., Miller R.E. and Wilkin P., Phylogenetic analysis of Ipomoea, Argyreia, Stictocardia and Turbina suggests a generalized model of morphological evolution in morning glories, Syst Bot.,26, 585-602 (2001)
- Laemmli U.K., Cleavage of structural proteins during assembly of the head of bacteriophage T4, Nature, 227, 680-685 (1970)
- Bradford M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding, Anal. Biochem., 72, 248-254 (1976)
- Pavlicek A., Hrda S. and Flegr J., Free tree free ware program for construction of phylogenetic trees on the basis of distance data and bootstrap/Jack Knife analysis of the tree robustness, Application in the RAPD analysis of genus Frenkelia, Folia Biologiica., 45, 97-99 (1999)
- Jaccard P., Nouvelles rescherches sur la distribution florale, Bull. Soc. Vaud. Sci. Nat., 44, 223-270 (1908)
- Roderic D.C., Treeview 32, Versionl. Retrieved from: http:// taxonomy, zoology, gla. ac. uk. /rod/rod. Html., (2001)