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Genomic Discrimination of Eleven Commercial Mushrooms by DNA Fingerprinting using RAPD Marker

Author Affiliations

  • 1Sangenomics Research Labs, 16, 2nd Cross, Krishna Reddy Colony, Domlur Layout, Airport Road, Bangalore- 560071, INDIA

Int. Res. J. Biological Sci., Volume 2, Issue (10), Pages 1-5, October,10 (2013)

Abstract

In today’s world with burgeoning population the growth cultivation of edible mushrooms holds great significance because of their nutritive and therapeutic properties. The advances in research on mushroom breeding and production are highly constrained compared to other crops; due to lack of previous knowledge in mushroom genetic. Present study focuses on establishing phylogenetic relationship among the eleven elite edible mushrooms using RAPD markers. Eleven mushroom samples namely 1) A. bisporus (Button), 2) A. bisporus (Portobello), 3) P. eryngii (King Oyster), 4) L. edodes (shiitake), 5) H. tessellatus (Brown Shimeji), 6) H. tessellatus (White Shimeji), 7) F. velutipes (Enoki), 8) P. ostreatus (Oyster), 9) P. djamor (Pink Oyster), 10) C. indica (Milky), and 11) P. florida (Florida Oyster) were used. Molecular markers serve as quick and reliable tool to establish the identities of mushrooms and are helpful in mushroom taxonomy. Among the various primers used to OPZ 10 gave the most distinguished and scorable band pattern. To estimate the similarity and genetic distance among different mushroom, cluster analysis based on frequency similarity was performed. The RAPD analysis in this study has proven to be useful in discrimination, characterization and differentiation of the fungal cultivars.

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