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Diagnosis of Toxoplasmosis in HIV/AIDS patients with Immunoblotting

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Author Affiliations

  • 1 Department of Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (8), Pages 61-64, December,10 (2012)

Abstract

Toxoplasmosis is a common parasitic illness affecting humans. Reactivation of latent infection in HIV infected patients can cause encephalitis. Diagnosing the disease rests on serological methods. ELISA for IgG antibodies remains positive in most HIV-infected patients with toxoplasmosis while immunoblotting can predict the development of encephalitis. Immunodiagnosis by urine sample is easy and has been successfully tried in other parasitoses. So this study aimed at detection of antitoxoplasma antibodies by immunoblotting in sera and urine of HIV infected patients. In patients tested positive for antitoxoplasma IgG, the 97 kDa and 53 kDa antigenic bands were the most reactive in serum and urine respectively.These antigens can be purified and used for immunodiagnosis by ELISA and similar procedures to increase the specificity of diagnosis.

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