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Desoxirrobonuclease Activity in Clinical Mycoplasma fermentans Isolate

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Author Affiliations

  • 1Centro de Investigaciones en Ciencias Microbiológicas del Instituto de Ciencias de la Benemérita Universidad Autónoma de Puebla, MÉXICO
  • 2 Centro de Química del Instituto de Ciencias de la Benemérita Universidad Autónoma de Puebla, MÉXICO
  • 3 Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional, MÉXICO

Int. Res. J. Biological Sci., Volume 1, Issue (8), Pages 82-84, December,10 (2012)

Abstract

Mycoplasmas are a heterogeneous group of the smallest organisms capable of self replication, some mycoplasmas cause respiratory or urogenital diseases in humans. However, mycoplasmas often chronically colonize our respiratory and urogenital tracts without apparent clinical significance. In this respect, wall free mycoplasmas are among the few prokaryotes that can grow silently in close interaction with mammalian cells for a long period of time. However, prolonged interactions with mycoplasmas of seemingly low virulence could, through a gradual and progressive course, significantly affect many biologic properties of mammalian cells. DNases are important constituents of mycoplasmal membranes and may be involved in the acquisition of host nucleic acids required for growth. The objective was detected DNase activity in clinical Mycoplasma fermentans isolate. Digestion products were analyzed by loading a Tris-borate agarose gel, absorbance and medium for DNase test. DNase activity was observed in strains tested and the levels of activity varied, Mycoplasma fermentans P140 showing greater activity. Results suggest DNase activities may be essential for growth and survival, this finding suggest that there is a mechanism of nucleotide acquisition.

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