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Role of Neem (Azardirachta indica) as a Plant extract Dewormer for Ancylostoma caninum Infection in Mice

Author Affiliations

  • 1 School of Studies in Zoology and Biotechnology, Vikram University, Ujjain–456010, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (7), Pages 44-48, November,10 (2012)

Abstract

Gastrointestinal parasite is serious threat to the productivity of livestock in developing nation. The major mechanism of controlling nematode parasite of livestock has been limited to the use of synthetic dewormer. Several plant products have been exploited for their dewormer activity. Neem has been shown to possess many medicinal properties including dewormer property. The purpose of this experiment was to study the dewormer activity of neem against A. caninum in infected mice. Two groups of mice were infected with A. caninum infective larvae. Before infection one group of mice were given neem extract at dose level of 0.2ml/ mouse. One group of mice served as non treated group. The dewormer activity was determined by larval reduction, mast cell and eosinophil cell level. Neem extract were highly effective in reducing the number of A. caninum. Larval reduction showed that the number of larvae reduced was higher in the treated and infected group compared to the infected group within 72 and 96 hours after challenge infection. Mast cell result suggest that on day 16 and 24 in mice infected with A. caninum larvae developed higher mastocytosis in comparison to treated and infected group. Decline level of eosinophil cell recorded on day 16 and 24 in treated and infected group when compared with infected group. The result suggests that the number of larvae correlated with number of mast cells and eosinophil cell and a potential role of neem extract as a dewormer activity against A. caninum in mice.

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