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Antioxidant Activity and Phenolic Compounds of Vitex Trifolia Var, Simplicifolia Associated with Anticancer

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Author Affiliations

  • 1Universiti Malaysia Kelantan, Locked Bag No 100, 17600 Jeli, Kelantan, MALAYSIA Forest Research Institute Malaysia, 52110 Kepong, Selangor, MALAYSIA

Int. Res. J. Biological Sci., Volume 1, Issue (3), Pages 65-68, July,10 (2012)

Abstract

Vitex trifolia var.simplicifolia plant was found growing wildly at the beach, especially at Pantai Sri Tujuh, Tumpat, Kelantan, Malaysia. The extracted leaves were commonly used to prepare traditional dessert among Siamese communities in Kelantan called “Khanom Bai Kunthi” for a long time. Water was used as an extraction agent by the peoples in this area. This extracted leaves were used by the Siamese communities in Tumpat area because it has natural color, flavor and fragrance properties. The Siamese communities who consumed “Khnom Bai Kunthi” was found to be healthy life and live longer. This is the starting point of the study. For the purpose of this study, Vitex trifolia var.simplicifolia leaves were extracted by using 80% methanol in the laboratory. The extracted V. trifolia var.simplicifolia was analyzed and found to be high in antioxidant activity of phenolic compound. The result shows that the extracted V. trifolia var. simplicifolia had high amount of total phenolic compound (44.66µg of GAE/mg of fresh weight). The plants secondary metabolites normally play role in cancer treatment. Cytotoxicity activity of the extracted V. trifolia var.simplicifolia was evaluated on MCF-7 (Breast cancer cell line), HT-29 (colorectal cancer cell line), and WRL-68 (normal liver cell line), and the results show that, cytotoxicity activity (IC50 values 78.87µg/ml, 77.50µg/ml, and 78.29µg/ml, respectively).

References

  1. Chaitra H., Madhuri M., Nitisha S.T., Arijit D., Sourav B. and Rohit, K.C., Evaluation of Antimicrobial Properties, Phytochemical Contents and Antioxidant Capacities of Leaf Extracts of Punica granatum L., ISCA Journal of Biological Sciences, 1(2), 32-37 (2012)
  2. Verma V., Bhardwaj A., Rathi S. and Raja R.B., A Potential Antimicrobial Agent from Cocos nucifera mesocarp extract; Development of a New Generation Antibiotic, ISCA Journal of Biological Sciences, 1(2), 48-54 (2012)
  3. Shaziy B. and Goyal P.K., Anthelmintic effect of Natural Plant (Carica papaya) extract against the Gastrointestinal nematode, Ancylostoma caninum in Mice, ISCA Journal of Biological Sciences, 1(1), 2-6 (2012)
  4. Kanife U.C., Odesanmi O.S., Adekumle A.A. and Doherty V.F., Effects of Ethanol Extracts of Healthy and Infected Panicum maximum (Jacq.) Floret on liver and kidney function profile and histopathology in Sprague-dawley rats, Research Journal of Recent Sciences, 1(5), 8-13 (2012)
  5. Bobade S.N. and Khyade V.B., Influence of Inorganic Nutrients on the activity of Enzyme, Nitrate reductase in the leaves of Mulberry, Morus alba (L) (M-5 variety), Research Journal of Recent Sciences, 1(5), 14-21 (2012)
  6. Khyade V.B., Shukla K.K. and Sarawade J.P., Juvenoid activity of some non mulberry plant extractives through inhibition of chitin deposition in the integument of fifth instar larvae of silkworm, Bombyx mori (L) (Race: PM x CSR2), Research Journal of Recent Sciences, 1(ISC-2011)6-6 (2012)
  7. Jeetendra S. and Dilip Kumar A., Ethno Medicinal Plants used by Tribal Communities for the Treatment of Snakebite in West Nimar, MP, India, ISCA Journal of Biological Sciences, 1(2), 77- 79 (2012)
  8. Muthusamy P., Murugan S. and Smitha M., Removal of Nickel ion from Industrial Waste Water Using Maize Cob, ISCA Journal of Biological Sciences, 1(2), 7- 11 (2012)
  9. Aweng E.R., Anwar A.I., Siti Rafiqah M.I. and Suhaimi O., Cassia alata as a Potential Coagulant in Water Treatment, Research Journal of Recent Sciences, 1(2), 28-33 (2012)
  10. Mangale Sapana M., Chonde Sonal G. and Raut P.D., Use of Moringa oleifera (Drumstick) seed as Natural Absorbent and an Antimicrobial agent for Ground Water Treatment, Research Journal of Recent Sciences, 1(3), 31-40 (2012)
  11. Salah EL-Kousy, Mona Mohamed and Shimaa Mohamed, Phenolic and biological activities of Vitex trifolia aerials parts, Life Science Journal, 9(2), 670-677 (2012)
  12. Thompson L., What Substance Gives Plants Their Green Color? http:wwwwehow.com/how-does 5470613 substance-gives-plants-green-color.html [12 October 2010] (2009)
  13. Blakesley K., Peak Fall Colors:Why Do Leaves Change Color? http://webchace.googleusercontent.com/search?q=cache:yXAaAmRgvSQJ:www.associatedcontent.com/article/224519/peak fall colors why do leaveschange.html+factor+destroying+chlorophyll&cd=1&cd=1&hl=en&ct=clnk&client=firefox-a [12 October 2010] (2009)
  14. Holcorft D.M. and Kader A.A., Carbon Dioxide- Induced Changes in color and Anthocyanin Synthesis of Stored Strawberry Fruit, HortScience, 36(7), 1244-1248 (1999)
  15. Romero C. and Bakker R., Interaction Between Grape Anthocyanins and Pyruvic Acid, with Effect of pH and Acid Concentration on Anthocyanin Composition and Color in Model Solutions Interaction, Journal of Agriculture Food Chemical, 47(8), 3130-3139 (1999)
  16. Huang S., Pettaway C.A., Uehara H., Bucana C.D. and Fidler I.J., Blockade of NF-kB activity in human prostate cancer cells is associated with suppression of angiogenesis, invasion and metastasis, Oncogene,20, 4188-4197 (2001)
  17. Cai Y., Luo Q., Sun M. and Corke H., Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer, Life Sciences, 74, 2157-2184 (2003)
  18. Singleton V.L. and Rossi J.A., Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, American Journal of Enology and Viticulture, 16, 144-158 (1965)
  19. Pikarsky E. Porat R.M., Stein I., Bramovitch R.A., Amit S., Kasem S., Gutkovich-Pyest E., Urieli-Shoval S., Galun E. and Ben-Neriah Y., NF-kappaB functions as a tumour promoter in inflammation-associated cancer, Nature, 431, 461- 466 (2004)
  20. Lin A. and Karin M., NF-kB in Cancer: a Market Target, Semin, Cancer Biology13, 107-114 (2003)
  21. Henriquez C., Almonacid S., Chiffelle I., Valenzuela T., Araya M., Cebezas L., Simpson R. and Speisky H., Determination of Antioxidant Capacity, Total Phenolic Content and Mineral Composition of Different Fruit Tissue of Five Apple Cultivars Grown in Chile, Chilean Journal of Agricultural Resources, 70(4), 523-536 (2010)
  22. Osorio-Esquivel O., Ortiz-Moreno A., Alvarez A.B., Dorora-Alvarez L. and Giuti M.M., Phenolics betacyanins and antioxidant activity in Opuntia joconostle fruits, Food Research International, 44(7), 2160-2168 (2011)
  23. Wu L.C., Hsu H.W., Chen Y.C., Chiu C.C., Lin Y.I. and Ho J.A., Antioxidant and anti proliferative activities of red pitaya, Food Chemistry, 95(2), 319-327 (2006)
  24. Gungor N. and Sengul M., Antioxidant Activity, Total Phenolic Content and Selected Physicochemical Properties of White Mulberry (Morus Alba L.) Fruits, International Journal of Food Properties, 11(1), 44-52 (2008)