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Potential Antimicrobial Agent from Cocos nucifera mesocarp extract; Development of a New Generation Antibiotic

Author Affiliations

  • 1 Department of Biotechnology, National Institute of Technology, Raipur-492010, INDIA
  • 2Department of Biotechnology, National Institute of Technology, Raipur-492010, INDIA
  • 3 Department of Biotechnology, National Institute of Technology, Raipur-492010, INDIA
  • 4 Presently Molecular Sciences and Nanotechnology, College of Eng. and Science, Louisiana Tech University, Ruston 71270 USA

Int. Res. J. Biological Sci., Volume 1, Issue (2), Pages 48-54, June,10 (2012)

Abstract

Cocos nuciferamesocarp has been used from time immemorial in the treatment of various skin ailments. Cocos nuciferamesocarp extract can also be used as an antimicrobial agent against clinical pathogens. The purpose of this study was to confirm the anti-bacterial effect of cocos nuciferamesocarp powder using escherichia coli and salmonella typhi. For this six different solvent extracts of cocos nuciferamesocarp powder were produced and the anti-bacterial activity was determined using disc diffusion method. The specific therapeutic compounds were isolated using thin layer chromatography (TLC) and qualitative analysis was performed by high performance liquid chromatogaphy (HPLC) and fourier transform infra-red spectroscopy (FTIR). The antimicrobial activity was found to be highest in case of benzene solvent against E.coli. In the case of salmonella typhi the antimicrobial activity was found to be highest with diethyl ether. TLC provided two fractions of the coconut shell extract powderwhichwere eluted further. FTIR graphs provided characteristic peaks which represented the components responsible for antimicrobial activity. HPLC helped to identify the active biocomponents as tocopherol, palmitoleyl alcohol, cycloartanol and -sitosterol. The Cocos nuciferamesocarp powder can be utilized to develop indigenous antibiotics which can replace conventional antibiotics.

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