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Evaluation of Antimicrobial Properties, Phytochemical Contents and Antioxidant Capacities of Leaf Extracts of Punica granatum L.

Author Affiliations

  • 1Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA
  • 2Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA
  • 3Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA
  • 4Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA
  • 5Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA
  • 6Sapthagiri College of Engineering, Bangalore, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (2), Pages 32-37, June,10 (2012)

Abstract

The numerous side effects associated with the use of allopathic drugs have led to renewed level of interest in Ayurvedic medicines. Punica granatum L. is a fruit-bearing deciduous shrub, belonging to the family Lythraceae and is cultivated throughout Asia, Middle-East and the Mediterranean region. The shrub has been known to possess several medicinal and curative properties. The present investigation focuses on antimicrobial properties, phytochemical analysis and antioxidant potential of leaf extracts of Punica granatum L. The methanolic extract inhibited Staphylococcus aureus, Bacillus cereus, Salmonella typhi and Proteus mirabilis, whereas, the chloroform, ethyl acetate and aqueous extracts exhibited moderate inhibitory effects against the test bacteria. On the other hand, only methanolic extract demonstrated antifungal activity against Aspergillus niger, Aspergillus flavus, Trichophyton rubrum, Candida albicans and Cryptococcus sp. The phytochemical screening of the methanolic extract of the leaves revealed the presence of carbohydrates, reducing sugars, sterols, glycosides, phenolics, tannins, flavonoids, proteins and saponins, whereas, gums were not detected. Total antioxidant potential of the methanolic and aqueous extracts were found as 2.26 and 1.06 mg of ascorbic acid equivalent per ml of the extract, respectively. The results indicated that the methanolic extract of the leaves are pharmacologically more active than the other extracts.

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