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Evaluation of Antimicrobial Properties, Phytochemical Contents and Antioxidant Capacities of Leaf Extracts of Punica granatum L.

Author Affiliations

  • 1Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA
  • 2Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA
  • 3Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA
  • 4Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA
  • 5Dept. of Microbiology, A Div. of Centre for Advanced Studies in Biosciences, Jain University, Bangalore, INDIA
  • 6Sapthagiri College of Engineering, Bangalore, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (2), Pages 32-37, June,10 (2012)


The numerous side effects associated with the use of allopathic drugs have led to renewed level of interest in Ayurvedic medicines. Punica granatum L. is a fruit-bearing deciduous shrub, belonging to the family Lythraceae and is cultivated throughout Asia, Middle-East and the Mediterranean region. The shrub has been known to possess several medicinal and curative properties. The present investigation focuses on antimicrobial properties, phytochemical analysis and antioxidant potential of leaf extracts of Punica granatum L. The methanolic extract inhibited Staphylococcus aureus, Bacillus cereus, Salmonella typhi and Proteus mirabilis, whereas, the chloroform, ethyl acetate and aqueous extracts exhibited moderate inhibitory effects against the test bacteria. On the other hand, only methanolic extract demonstrated antifungal activity against Aspergillus niger, Aspergillus flavus, Trichophyton rubrum, Candida albicans and Cryptococcus sp. The phytochemical screening of the methanolic extract of the leaves revealed the presence of carbohydrates, reducing sugars, sterols, glycosides, phenolics, tannins, flavonoids, proteins and saponins, whereas, gums were not detected. Total antioxidant potential of the methanolic and aqueous extracts were found as 2.26 and 1.06 mg of ascorbic acid equivalent per ml of the extract, respectively. The results indicated that the methanolic extract of the leaves are pharmacologically more active than the other extracts.


  1. Samy R.P., Pushparaj P.N. and Gopalakrishnakone P.A., Compilation of bioactive compounds from Ayurveda, Bioinformation, 3, 100–110 (2008)
  2. Ballabh B. and Chaurasia O.P., Traditional medicinal plants of cold desert Ladakh--used in treatment of cold, cough and fever, J. Ethnopharmacol., 112(2), 341-349 (2007)
  3. Okigbo R.N., Eme U.E. and Ogbogu S., Biodiversity and conservation of medicinal and aromatic plants in Africa, Biotechnol. Mol. Biol. Rev., 3(6), 127-134 (2008)
  4. Adesokan A.A., Yakubu M.T., Owoyele B.V., Akanji M.A., Soladoye A. and Lawal O.K., Effect of administration of aqueous and ethanolic extracts of Enantia chlorantha stem bark on brewer’s yeast-induced pyresis in rats, Afri. J. Biochem. Res., 2(7), 165-169(2008)
  5. Altuner E.M., Investigation of antimicrobial activity of Punica granatum L. fruit peel ash used for protection against skin infections as folk remedies especially after male Circumcision, Afr. J. Microbiol Res., 5(20), 3339-3342 (2011)
  6. Qnais E.Y., Elokda A.S., Abu Ghalyun Y.Y. and Abdulla F.A., Antidiarrheal activity of the aqueous extract of Punica granatum (Pomegranate) peels, Pharm. Biol., 45(9), 715–720 (2007)
  7. Arun N. and Singh D.P., Punica granatum: a review on pharmacological and therapeutic properties, IJPSR, 3(5),1240-1245 (2012)
  8. Choi J.G., Kang O.H., Lee Y.S., Chae H.S., Oh Y.C., Brice O.O., Kim M.S., Sohn D.H., Kim H.S., Park H., Shin D.W., Rho J.R. and Kwon D.Y., In vitro and in vivo antibacterial activity of Punica granatum peel ethanol extract against Salmonella, Evid. Based Complement, Alternat. Med., 1-8 (2011)
  9. Dahham S.S., Ali M.N., Tabassum H. and Khan M., Studies on antibacterial and antifungal activity of pomegranate (Punica granatum L.), American-Eurasian J. Agric. and Environ. Sci., 9(3), 273-281 (2010)
  10. Inabo H.I. and Fathuddin M.M., In vivo antitrypanosomal potentials of ethyl acetate leaf extracts of Punica granatum against Trypanosoma brucei brucei, Adv. Agr. Bio., 1, 82-88 (2011)
  11. Moussa A.M., Emam A.M., Diab Y.M., Mahmoud M.E. and Mahmoud A.S., Evaluation of antioxidant potential of 124 Egyptian plants with emphasis on the action of Punica granatum leaf extract on rats, IFRJ, 18, 535-542(2011)
  12. Prakash C.V.S. and Prakash I., Bioactive chemical constituents from pomegranate (Punica granatum) juice, seed and peel-a review, Int. J. Res. Chem. Environ., 1(1),1-18 (2011)
  13. Bauer A.W., Kirby W.M.M., Sherris J.C. and Turck M., Antibiotic susceptibility testing by a standardized single disk method, Amer. J. Clin. Pathol., 45, 493-496 (1966)
  14. Ramkrishnan S. and Rajan R., Text Book of Medical Biochemistry, Orient Longman, New Delhi, India (1994)
  15. Evans W.C., Trease and Evans Pharmacology, (14th Edn), Harcourt Brace and Company, Asia. Pvt. Ltd., Singapore (1997)
  16. Kokate C.K., Practical Pharmacognosy, (4th Edn), Vallabh Prakashan Publication, New Delhi, India (1999)
  17. Mace M.E., Histochemical localization of phenols in healthy and diseased tomato roots, Phytochem., 16, 915-925 (1963)
  18. Yasuma A. and Ichikawa T., Ninhydrin-Schiff and alloxan-Schiff staining; a new histochemical staining method for protein, J. Lab. Clin. Med., 41(2), 296–299(1953)
  19. Prieto P., Pineda M. and Aguilar M., Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E, Anal. Biochem., 269, 337-341 (1999)
  20. Dev S., Ethnotherapeutics and modern drug development: the potential of Ayurveda, Curr. Sci.,73(11), 909-928 (1997)
  21. Cox P.A. and Balick M.J., The ethnobotanical approach to drug discovery, Sci. Am., 270, 60-65 (1994)
  22. Yasoubi P., Barzegar M., Sahari M.A. and Azizi M.H., Total phenolic contents and antioxidant activity of pomegranate (Punica granatum L.) peel extracts, J. Agric. Sci. Technol., 9, 35-42 (2007)
  23. Rathinamoorthy R., Udayakumar S. and Thilagavathi G., Antibacterial efficacy analysis of Punica granatum L. leaf, rind and Terminalia chebula fruit extract treated cotton fabric against five most common human pathogenic bacteria, Int. J. of Pharm. and Life Sci., 2(10),1147-1153 (2011)
  24. Braga L.C., Leite A.A., Xavier K.G., Takahashi J.A., Bemquerer M.P., Chartone-Souza E. and Nascimento A.M., Synergic interaction between pomegranate extract and antibiotics against Staphylococcus aureus, Can. J. Microbiol., 51, 541-547 (2005)
  25. Silva M.A.R., Higino J.S., Pereira J.V., J.P. SiqueiraJúnior and M.S.V. Pereira, Antibiotic activity of the extract of Punica granatum Linn, Over bovine strains of Staphylococcus aureus, Brazilian J. Pharmacog., 18(2),209-212 (2008)
  26. Machado T.B., Pinto A.V., Pinto M.C.F.R., Leal I.C.R., Silva M.G., Amaral A.C.F., Kuster R.M. and NettodosSantos K.R., In vitro activity of Brazilian medicinal plants, naturally occurring naphthoquinones and their analogues, against methicillin-resistant Staphylococcus aureus, Int. J. Antimicrob. Agents, 21, 279-284 (2003)
  27. Mathabe M.C., Nikolova R.V., Lall N. and Nyazema N.Z., Antibacterial activities of medicinal plants used for the treatment of diarrhoea in Limpopo Province, South Africa, J. Ethnopharmacol., 105, 286-293 (2005)
  28. Elmanama A.A., Alyazji A.A. and Gheneima N.A.A., Antibacterial, antifungal and synergistic effect of Lawsonia inermis, Punica granatum and Hibiscus sabdariffa, Annals of Alquds Medicine, 7, 33-41 (2011)
  29. Reddy M.K., Gupta S.K., Jacob M.R., Khan S.I. and Ferreira D., Antioxidant, antimalarial and antimicrobial activities of tannin-rich fractions, ellagitannins and phenolic acids from Punica granatum L., Planta Med.,73, 461- 467 (2007)
  30. Glazebrook J. and Ausubel F.M., Isolation of phytoalexin-deficient mutants of Arabidopsis thaliana and characterization of their interactions with bacterial pathogens, Proc. Natl. Acad. Sci. USA, 91(19), 8955–8959 (1994)
  31. Egharevba H.O., Kunle O.F., Iliya I., Orji P.N., Abdullahi M.S., Okwute S.K. and Okogun J.I., Phytochemical analysis and antimicrobial activity of Punica granatum L. (fruit bark and leaves), New York Science Journal, 3(12), 91-98 (2010)
  32. Jain P. and Nafis G., Antifungal activity and phytochemical analysis of aqueous extracts of Ricinus communis and Punica granatum, JPR, 4(1), 128-129(2011)
  33. Wang R., Ding Y., Liu R., Xiang L. and Du L., Pomegranate: Constituents, bioactivities and pharmacokinetics, Fruit, Veg. Cereal Sci. Biotech., 4(2),77-87 (2010)
  34. Prasad R.N., Viswanathan S., Devi J.R., Nayak V., Swetha V.C., Archana B.R. Parathasarathy N. and Rajkumar J., Preliminary phytochemical screening and antimicrobial activity of Samanea saman, J. Med. Plants Res., 2, 268-270 (2008)
  35. Jamine R., Daisy P. and Selvakumarb B.N., In vitro efficacy of flavonoids from Eugenia jambolana seeds against ESAYL-producing multidrug-resistant enteric bacteria, Res. J. Microbiol., 2, 369-374 (2007)
  36. Sumino M., Sekin T., Ruanagrungsi N., Igarashi K. and Ikkegami F., Ardisiphenols and other antioxidant principles from the fruits of Ardisia colorata, Chem. Pharm. Bull., 50, 1484-1487 (2002)
  37. Lodovici M., Guglielmi F., Meoni M. and Dolara P., Effect of natural phenolic acids on DNA oxidation in vitro, Food Chem. Toxicol., 39(12), 1205-1210 (2001)
  38. Valentao P., Fernandes E., Carvalho F., Andrade P.B., Seabra R.M. and Bastos M.,, Antioxidative properites of carbon Cynara cardunculus L. infusion against superoxide radical hydroxy radical and hypochlorous acid, J. Agric. Food Chem., 50, 4989-4993 (2002)