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Fungal Degradation of Azo dye- Red 3BN and Optimization of Physico-Chemical Parameters

Author Affiliations

  • 1Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Bangalore-560 090, INDIA
  • 2Department of Biotechnology, Acharya Institute of Technology, Soladevanahalli, Bangalore-560 090, INDIA

Int. Res. J. Biological Sci., Volume 1, Issue (2), Pages 17-24, June,10 (2012)


Decolorization of azo dye red 3BN by three fungal species Penicillium chrysogenum, Aspergillus niger and Cladosporium sp. has been analyzed using potato dextrose agar (PDA) medium containing 0.01% of Red 3 BN. Physico-chemical parameters like carbon source, nitrogen source, temperature, pH and inoculum volume are optimized for the decolorization process by changing one parameter at a time. Optimal condition for P. chrysogenum was found to be 1% maltose 1% yeast extract, pH 8, 27�C and 2% inoculums. Ideal condition for A. niger was found to be1% maltose , 1% yeast extract, pH 8, 27C and 10% inoculum and that for Cladosporium sp. was found to be1% maltose, 1% peptone, pH 6, 37C and 10% inoculum . Extent of decolorization recorded by P. chrysogenum under ideal conditions was 99.56%, A. niger was 98.64% and that by Cladosporium sp. was 98.18%. The study has confirmed the potential of the above fungi in the decolorization of azo dye Red 3BN and opened scope for future analysis of their performance in the treatment of textile effluent.


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