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Morphological and morphometrical analyses of Blood Cells of Emus (Dromaius novaehollandiae) in Captive condition

Author Affiliations

  • 1State Forensic Science Laboratory, Bhubaneswar, Odisha, India

Res. J. Forensic Sci., Volume 8, Issue (2), Pages 1-8, July,29 (2020)

Abstract

The designed of the study was made for analyses of morphology and morphometry (i.e., measurement) of cells of blood of emus in captive condition. Since studies on morphological features of erythrocytes and leucocytes of captive emus were not properly documented in literature, to know the various shapes of erythrocytes (RBCs) and leucocytes of them and to study morphometry of blood cells in four different months this work is undertaken. For observance of cellular morphology of erythrocytes and leukocytes in them total fourteen emus were taken for the present study. From the jugular veins sample blood were taken by an expert from one private farm of Bhubaneswar, Odisha, India and transferred into EDTA vials and blood smears were made on clean microscopic slides. Then staining of blood smears were done with Giemsa stain to study the cellular morphology. The findings of the study reveal that captive emus have various shapes of erythrocytes and by this type of study shapes of normal erythrocyte and abnormal erythrocytes can be known. RBCs and their nuclei and leucocytes were photomicrographed and observed below the microscope under 40X objective. Length and breadth of RBCs and their nuclei, lymphocytes and their nuclei and other leucocytes were measured with the help of ocular micrometer and stage micrometer. Mean and standard error were calculated for statistical analyses.

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