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Diffrential Pulses Adsorptive Stripping Voltammetry (DPSAV) Determination of Mercury (HG) in Blood Using Gold Rotating Disc Electrode(RDE)

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Author Affiliations

  • 1Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi-110029, INDIA
  • 2Department of Biotechnology, Kumaun University Nainital Uttarakhand, INDIA
  • 3Department of Biochemistry, University of Delhi South Campus, New Delhi- 110021, INDIA

Res. J. Forensic Sci., Volume 2, Issue (2), Pages 1-4, April,29 (2014)

Abstract

The salts of mercury are of great toxicological importance and can cause poisoning, therefore quantitative determination of traces of mercury in blood is very essential. Routinely, inductive coupled plasma, graphite furnace atomic absorption spectrometry are used for analysis. An attempt has been made to develop new method for determination of traces of mercury in blood using differential- pulse adsorptive stripping voltametry (DPASV). The analysis utilizes three electrode systems, Rotating Disc Electrode (RDE) as a working electrode, Ag/AgCl (filled with ammonium buffer) as a reference electrode and glassy carbon electrode as an auxiliary electrode. Blood was processed by closed digestion method using 34.5% Nitric acid (HNOз). Determination of mercury was made by primary solution with a sweep rate of 20 mv/s and pulse amplitude 50 mv by standard addition method. The solution was purged with nitrogen gas and cleaning was done at 1300 mv for 30 sec. and the potential was scanned from 500 mv to 850 mv on RDE with stirrer speed 2000 rpm. The mercury ions were deposited by adsorption at 370 mv for 60 sec. The deposited metal was stripped by scanning the potential from 500 mv to 850 mv using adsorptive stripping mode. The stripping current arising was correlated with the concentration of the metal in the sample. The peak potential for mercury is 640 mv .The detection limit of mercury by this method was 1.0 ug/l.

References

  1. Keating M.H., Mahaffey K.R., Schoemy R., Mercury Study Report to Congress, Executive Summary Washington, D.C.: Environmental Protection Agency: EPA, (452), R-97-003 (1997)
  2. Wierzbicki R., Prazanowski M., Michalska M., Disorders in blood coagulation in humans occupationally exposed to mercuric vapors, J Trace Elem Exp Med, (29), 15-21 (2001)
  3. Richard D.H.S., Application of voltametry in environment science, Environmental pollution, (10), 261-265 (1985)
  4. Huston M.C., Role of mercury hypertension, cardiovascular disease and stroke, J. Clinical hypertension (8), 621-626 (2011)
  5. Vogel A.I., A Textbook of Quantitative Inorganic Analysis, The ELBS and Longman, Publishing CO. London, (3rd Edn), (1975)
  6. Guan, Dai H., chelation therapy for mercury Poisoning, Biochemistry insight, (2), 63-66 (2009)
  7. Nixon D.E., Burritt M.F. and Moyer T.P., The Determination of mercury in whole blood and urine by inductively coupled plasma mass spectrometry, Spectrochimica Acta, (54), 1141-1153 (1999)
  8. Hultberg B., Anderson A. and Isaksson A., Interaction of metals and thiols in cell damage and glutathione distribution: potentition of mercury toxicity by dithiothreitol, Toxicology, (100), 156: 93 (2001)
  9. Mather TA., Pyle DM., Volcanic emissions of mercury to the atmosphere: global and regional inventories, Science Total Environment, (9), 327-323 (2004)
  10. Yorifugi T., Tsuda T., Kashima S., et al. Long-term exposure to methyl mercury and its effects on hypertension in Minamata, Environ Res. (110), 40–46 (2010)