6th International Young Scientist Congress (IYSC-2020) will be Postponed to 8th and 9th May 2021 Due to COVID-19. 10th International Science Congress (ISC-2020).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Omega-3 poly unsaturated fatty acids improves semen quality in adult male rats

Author Affiliations

  • 1Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
  • 2Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
  • 3Department of Physiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, Egypt
  • 4B.V.Sc. in science, Ainshams University, Egypt

Res. J. Animal, Veterinary and Fishery Sci., Volume 8, Issue (1), Pages 1-7, May,24 (2020)

Abstract

In order to assess the effect of Omega-3 PUNFs on the quality of semen in albino rats in order to get benefit from its use. Fifty mature male albino rats weighing 250-300g and 4-6 months of age were allocated randomly into 2 equal groups; group A that kept as control (n=25) and group B (n=25) where, rats subjected to oral administration of omega-3 for 2 months at 375mg/kg body weight daily. Semen was collected from both groups after 8 weeks for detecting the sperm motility, count and abnormalities. Sera were used for determination of testosterone level. In addition, testis homogenate was used for measuring catalase and malondialdehyde activity. The body weight and sex organs (testis, prostate gland and seminal vesicles) weights were recorded in both groups. Histological examination of testis was performed. We found a significant increase in sperm motility, count, testosterone and catalase concentrations with a decrease in sperm abnormalities percentage and malondialdehyde concentration in omega-3treated group as compared with control. No change in both body and sex organs weights was observed in treated group. In addition, numerous sperms in the lumen of the seminiferous tubules with numerous active spermatocytes were observed in the testis of omega-3treated group. It was concluded that Omega-3PUNFs had a positive and useful effects on the male fertility and could improve semen quality.

References

  1. Toshimori K., Ito, C., Maekawa, M., Toyama, Y., Suzuki-Toyota, F., et al. (2004)., Impairment of spermatogenesis leading to infertility., Anat. Scilnt., 79, 101-111.
  2. Austin, D. F. (1991)., Ipomoea littoralis (Convolvulaceae)-taxonomy, distribution, and ethnobotany., Economic Botany, 45(2), 251-256.
  3. Giahi, L., Mohammadmoradi, S., Javidan, A., & Sadeghi, M. R. (2016)., Nutritional modifications in male infertility: a systematic review covering 2 decades., Nutrition reviews, 74(2), 118-130.
  4. Saleh, R. A., & HCLD, A. A. (2002)., Oxidative stress and male infertility: from research bench to clinical practice., Journal of andrology, 23(6), 737-752.
  5. Esmaeili, V., Shahverdi, A. H., Moghadasian, M. H., & Alizadeh, A. R. (2015)., Dietary fatty acids affect semen quality: a review., Andrology, 3(3), 450-461.
  6. Brinsko, S. P., Varner, D. D., Love, C. C., Blanchard, T. L., Day, B. C., & Wilson, M. E. (2005)., Effect of feeding a DHA-enriched nutriceutical on the quality of fresh, cooled and frozen stallion semen., Theriogenology, 63(5), 1519-1527.
  7. Scorletti E. and Byrne C.D. (2013)., Omega-3 fatty acids, hepatic lipid metabolism, and nonalcoholic fatty liver disease., Annual Review of Nutrition, 33(1), 231-48.
  8. Rizos E. C., Ntzani E. E., Bika E., Kostapanos M. S. and Elisaf M.S. (2012)., Association between omega-3 fatty acid supplementation and risk of major cardiovascular disease events A Systematic Review and Meta-analysis., JAMA, 308(10), 1024-1033.
  9. Seo, T., Blaner, W. S., & Deckelbaum, R. J. (2005)., Omega-3 fatty acids: molecular approaches to optimal biological outcomes., Current opinion in lipidology, 16(1), 11-18.
  10. Scaioli, E., Sartini, A., Bellanova, M., Campieri, M., Festi, D., Bazzoli, F., & Belluzzi, A. (2018)., Eicosapentaenoic acid reduces fecal levels of calprotectin and prevents relapse in patients with ulcerative colitis., Clinical Gastroenterology and Hepatology, 16(8), 1268-1275. https://doi.org/10.1016/j.cgh.2018.01.036.
  11. Morgan, D. R., Dixon, L. J., Hanratty, C. G., El-Sherbeeny, N., Hamilton, P. B., Mc Grath, L. T. & McVeigh, G. E. (2006)., Effects of dietary omega-3 fatty acid supplementation on endothelium-dependent vasodilation in patients with chronic heart failure., The American journal of cardiology, 97(4), 547-551.
  12. Uygur, R., Aktas, C., Tulubas, F., Uygur, E., Kanter, M. E. H. M. E. T., Erboga, M., & Ozen, O. A. (2014)., Protective effects of fish omega‐3 fatty acids on doxorubicin‐induced testicular apoptosis and oxidative damage in rats., Andrologia, 46(8), 917-926.
  13. Hosseini, B., Nourmohamadi, M., Hajipour, S., Taghizadeh, M., Asemi, Z., Keshavarz, S. A., & Jafarnejad, S. (2019)., The effect of omega-3 fatty acids, EPA, and/or DHA on male infertility: a systematic review and meta-analysis., Journal of dietary supplements, 16(2), 245-256.
  14. Wathes, D. C., Abayasekara, D. R. E., & Aitken, R. J. (2007)., Polyunsaturated fatty acids in male and female reproduction., Biology of reproduction, 77(2), 190-201.
  15. Saravana K.A., Bhagya D. K., Devi Vara Prasad M., Grace Mary P., Sujeeth K,S., Swathi M. (2011)., Evaluation of the Protective Effects of Omega-3 Fatty Acids against Methotrexate Induced Testicular Toxicity in Male Albino Mice., International Journal of Phytopharmacology, 2(2), 48-52.
  16. Bansal A.K., Bilaspuri G.S. (2009)., Impacts of Oxidative Stress and Antioxidants on Semen Functions., Animal Science Papers and Reports, 27(1), 5-14.
  17. National Research Coincil (NRC) (1995)., Nutrient Requirements of laboratory Animals fourth revised edition., Washington, D.C., National Academy press.
  18. Hafez, E. S. E. (1970)., Rabbits., Reproduction and breeding techniques for laboratory animals, 273-315.
  19. Sikka, S. C., Rajasekaran, M. A. H. A. D. E. V. A. N., & Hellstrom, W. J. (1995)., Role of oxidative stress and antioxidants in male infertility., Journal of andrology, 16(6), 464-468.
  20. Robb, G. W., Amann, R. P., & Killian, G. J. (1978)., Daily sperm production and epididymal sperm reserves of pubertal and adult rats., Reproduction, 54(1), 103-107.
  21. Bearden H. J. and Fuquay J. (1980)., Applied Animal Reproduction., Reston Publishing Company, Inc, Aprentice-Hall Company. Reston, Virginia.
  22. Buege, J. A., & Aust, S. D. (1978)., Microsomal lipid peroxidation., In Methods in enzymology, 52, 302-310, Academic Press.
  23. Tietz, N. M. (1994)., Fundamentals of Clinical Chemistry., 2nd Edn WB Saunders. Philadelphia. pp, 692.
  24. Aebi H. (1984)., Measurement of catalase assay., Methods Enzymol, 105, 121-126.
  25. Fossati P. (1980)., Measurement of catalase assay., Clin. Chem., 26, 227-231.
  26. Janero, D. R. (1990)., Malondialdehyde and thiobarbituric acid-reactivity as diagnostic indices of lipid peroxidation and peroxidative tissue injury., Free radical biology and medicine, 9(6), 515-540.
  27. Suvarna S.K., Layton C. and Bancroft J. D. (2013)., Bancroft, 7th ed., Churchill Livingstone. Elsevier, England.
  28. Tamhane A. C., Dunlop D. D., (2000)., Statistic data analysis from Elementary to Intermediate., Upper Saddle River, USA. .
  29. Wathes, D.C, Abayasekara D. R and Aitken R. J. (2007)., Polyunsaturated fatty acids in male and female reproduction., Biol. Reprod., 77, 190-201.
  30. Estienne, M. J., Harper, A. F., & Crawford, R. J. (2008)., Dietary supplementation with a source of omega-3 fatty acids increases sperm number and the duration of ejaculation in boars., Theriogenology, 70(1), 70-76.
  31. Harris, M. A., Baumgard, L. H., Arns, M. J., & Webel, S. K. (2005)., Stallion spermatozoa membrane phospholipid dynamics following dietary n-3 supplementation., Animal reproduction science, 89(1-4), 234-237.
  32. Lin, D. S., Neuringer, M., & Connor, W. E. (2004)., Selective changes of docosahexaenoic acid-containing phospholipid molecular species in monkey testis during puberty., Journal of lipid research, 45(3), 529-535.
  33. Elelaimy, I. A., Elfiky, S. A., Hassan, A. M., Ibrahim, H. M., & Elsayad, R. I. (2012)., Genotoxicity of anticancer drug azathioprine (Imuran): role of omega-3 (ω-3) oil as protective agent., Journal of Applied Pharmaceutical Science, 2(4), 14-23.
  34. Yeste, M., Barrera, X., Coll, D., & Bonet, S. (2011)., The effects on boar sperm quality of dietary supplementation with omega-3 polyunsaturated fatty acids differ among porcine breeds., Theriogenology, 76(1), 184-196.
  35. Zararsiz, I., Kus, I., Davarci, M., Kus, M. A., Kaman, D., & Sarsilmaz, M. (2011)., The protective effects of omega-3 fatty acids on rat testicular tissue., Dicle Tip Dergisi, 38(4), 382.
  36. Nagata, C., Takatsuka, N., Kawakami, N., & Shimizu, H. (2000)., Relationships between types of Fat Consumed and Serum Estrogen and Androgen Concentrations in Japanese Men., Nutrition and cancer, 38(2), 163-167.
  37. De Catalfo, G. E. H., de Alaniz, M. J., & Marra, C. A. (2008)., Dietary lipids modify redox homeostasis and steroidogenic status in rat testis., Nutrition, 24(7-8), 717-726.
  38. Osman, A., Alsomait, H., Seshadri, S., El-Toukhy, T., & Khalaf, Y. (2015)., The effect of sperm DNA fragmentation on live birth rate after IVF or ICSI: a systematic review and meta-analysis., Reproductive biomedicine online, 30(2), 120-127.
  39. Gual-Frau, J., Abad, C., Amengual, M. J., Hannaoui, N., Checa, M. A., Ribas-Maynou, J. & Prats, J. (2015)., Oral antioxidant treatment partly improves integrity of human sperm DNA in infertile grade I varicocele patients., Human Fertility, 18(3), 225-229.
  40. Bestas, A., Bayar, M. K., Akpolat, N., & Okuducu, M. N. (2006)., Effect of sevoflurane anesthesia on the severity of renal histopathologic changes in rabbits pretreated with gentamicin: A controlled, investigator-blinded, experimental study., Current therapeutic research, 67(6), 386-395.
  41. Rezk, R. G., Abou Zaid, N. M., & Ahmed, A. G. (2011)., Role of Omega 3 fatty acids on radiation-induced oxidative and structural damage in different tissues of male albino rats., Journal of Radiation Research and Applied Sciences, 4(3A), 761-776.
  42. Safarinejad, M. R., Hosseini, S. Y., Dadkhah, F., & Asgari, M. A. (2010)., Relationship of omega-3 and omega-6 fatty acids with semen characteristics, and anti-oxidant status of seminal plasma: a comparison between fertile and infertile men., Clinical nutrition, 29(1), 100-105.
  43. Ahmed A. and Hoda M. N. (2009)., Evaluation of productive Effect of omega-3 and selenium on paraquat intoxicated rats., Slovak J. Anim. Sci., 42, (4), 180-187.
  44. Martínez-Soto, J. C., Domingo, J. C., Cordobilla, B., Nicolás, M., Fernández, L., Albero, P., & Landeras, J. (2016)., Dietary supplementation with docosahexaenoic acid (DHA) improves seminal antioxidant status and decreases sperm DNA fragmentation., Systems biology in reproductive medicine, 62(6), 387-395.
  45. Tabei S. M. B., Fakher S., Djalali, M., Javanbakht M. H., Zarei M., Derakhshanian H., Sadeghi M. R., Mostafavi E. and Kargar F. (2015)., Effect of vitamins A, E, C and omega-3 fatty acids supplementation on the level of catalase and superoxide dismutase activities in streptozotocin-induced diabetic rats Bratisl., Lek. Listy., 116(2), 115-118 .
  46. Meydan S., Altas M., Nacar A., Ozturk O.H., Tas U., Zararsiz I., Sarsilmaz M. (2012)., The protective effects of omega-3 fatty acid against toluene-induced neurotoxicity in prefrontal cortex of rats., Human Exp. Toxicol., 31(11), 1179-1185.
  47. Birsen O., Erdemir F., Parlaktas B.S. and Oezyurt H. (2008), Effects of omega-3 lipid peroxidation and antioxidant enzymes in MK-801 induced schizophrenic rat testis., Turkish J of Med Science, 38(4), 301-306.
  48. Castellano, C. A., Audet, I., Laforest, J. P., Matte, J. J., & Suh, M. (2011)., Fish oil diets alter the phospholipid balance, fatty acid composition, and steroid hormone concentrations in testes of adult pigs., Theriogenology, 76(6), 1134-1145.
  49. Suh M., Merrells K. J., Dick A. and Taylor C.G. (2011)., Testes of obese rats are highly responsive to n-3 long- chain fatty acids., Br. J. Nutr., 106(7), 1005-1012.
  50. Zidkova J., Sajdok J., Kontrova K., Kotrdova-Kozak A., Hanis T., Zidek V., Funcikova A. (2004)., Effects of oxidized dietary cod liver oil on the reproductive functions of Wistar rat., Czech J. Food Sci., 22, 108-120.
  51. Johnson, M., & Everitt, B. (1988)., The high concentration of glycogen in fetal cardiac muscle probably explains why the heart can maintain its contractile activity in the face of severe hypoxia., Essential reproduction, 3rd edn. Oxford: Blackwell Scientific, 275, 3140-3146.