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Detection of mecA Gene in Methicillin resistance Staphylococcus aureus (MRSA) from rural chickens, slaughter environment and personnel in Maiduguri, Borno State, Nigeria

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Author Affiliations

  • 1Department of Veterinary Medicine, University of Maiduguri, Nigeria
  • 2Department of Veterinary Medicine, University of Maiduguri, Nigeria
  • 3Department of Veterinary Microbiology, University of Maiduguri, Nigeria
  • 4Department of Veterinary Public Health and Preventive Medicine, University of Maiduguri, Nigeria
  • 5Department of Veterinary Medicine, University of Maiduguri, Nigeria
  • 6Department of Animal Health and Production, College of Agriculture Gujba, Yobe State, Nigeria

Res. J. Animal, Veterinary and Fishery Sci., Volume 6, Issue (2), Pages 1-7, March,24 (2018)

Abstract

Cross sectional study was carried out from Monday and Gamboru markets in Maiduguri, Nigeria to detect the presence of methicillin-resistant Staphylococcus aureus (MRSA) and determine the interplay between rural chickens, slaughter environment and personnel in the spread of MRSA. Samples were taken from skin of rural chickens, dressing tables, containers, knives and from the skin and nostrils of slaughter personnel. A total of 135 presumptive S. aureus isolates were analyzed and subjected to ORSAB test to identify MRSA using standard techniques. The test detected 57 (42.2%) MRSA isolates out of which 25 were randomly selected and subjected to PCR to detect S. aureus specific sequence gene and mecA gene. The isolates showed evidence of expression of S. aureus specific sequence gene with 107 bp targeted band whilst only 1 isolate was positive for mecA gene with a targeted band of 532 bp. It is therefore evident from this result that mecA gene is present in MRSA isolate from rural chicken which means transmission between human and animals is possible. Awareness programs should be implemented to educate the slaughter personnel and general public on its veterinary and public health importance. Therefore, these emphasize the need for further research to be carried out on the phylogenesis of MRSA in poultry and other livestock in Nigeria.

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