Genotoxic effects of Mercuric Chloride in the Albino Rat, Rattus norvegicus

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Genotoxic effects of Mercuric Chloride in the Albino Rat, Rattus norvegicus

Shaikh Irshad Ahmad

Author Affiliations

¹Department of Zoology, School of Life Sciences, Khandari Campus, Dr. B.R. Ambedkar University, Agra, UP, INDIA

Res. J. Animal, Veterinary and Fishery Sci., Volume 2, Issue (7), Pages 6-9, July,24 (2014)

Abstract

In the present study, toxicity of sublethal concentration of mercuric chloride on the karyotyping of Albino rat, Rattus norvegicus was observed, LD50 of mercury chloride was calculated and a sublethal concentration (1/20 LD50) was gives orally to the rat 20 hours prior to the colchicines treatment, 2 hours after this treatment the bone marrow was separated from the femur of test animal and collected in the test tube. The slide was prepared and studied under the microscope. The investigation reveals the numerical and structural aberrations in the chromosomes of the bone marrow cells in the test animal.

References

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[ref1] Harlt D.L. and Jones E.W., Analysis of genes and genomesJones and Barlet publishers Boston

[ref2] Russel P.J., Genetics, 5th edition, Addison Longman Inc, California, (1998)

[ref3] Clarkson T.W., The toxicology of mercury, Crit, Rev, Clin,Lab, Sci., 34, 369-403 (1997)
Google Scholar

[ref4] Grover P., Basu B.S., Dana D.K. and Begum S., In vivoxenotoxic effects mercuric chloride in rat peripheralleukocytes using comet assay, Toxicol., 167, 191-197(2001)

[ref5] Mark M.J., Jeans E.S. and Anthony D.W., Pretreatment andheavy metal LD50 values, Toxicol. Appl. Pharmacol., 49,41-44 (1979)
Google Scholar

[ref6] Heddle J.A., A rapid in vivo test for chromosome damage, Mutation Res., 18, 187-190 (1973)
Google Scholar

[ref7] Preston R.J., Dean B.J., Galloway S., Holden H., McFee A.and Shelby M., Mammalian in vitro cytogenetic assay ofchromosome aberration in bone marrow cells, MutationRes., 189, 157-165 (1987)
Google Scholar

[ref8] Szpirer et al., folia, Biol. (Praha), 42, 175-226 (1996)

[ref9] Levin, Nomenclature rules for rat chromosome G-bands, Hereditas, 77, 37-52 (1974)
Google Scholar

[ref10] Das S.K., Sharma A. and Talukdar G., Dose relatedclastogenic action of inorganic mercury, Nat. Acad. Sci.Leetters, 6, 153-156 (1983)
Google Scholar

[ref11] Deknudta G. and Gerber G.B., Chromosomal aberrationsin bone-marrow cells of mice given a normal or a calciumdeficientdiet supplemented with various heavy metals, Mutation Research/Genetic Toxicology, 68, 2163–168(1979)
Google Scholar

[ref12] Tarun G., Geeta T. and Archana S., Cytotoxicity of zincchloride in mice in vivo, Biological Trace ElementResearch, 30, 95-101 (1991)

[ref13] Bingham D., Bonner P.T., Cox R., Edwards A.A., GardinI., Haines J.W. and Harrison J.D., Comparison ofcytogenetic damage in cultured cells from cobalt-60gamma-radiation and the auger emitter zinc-65, Int. J.Radiat. Biol., 76, 1223-1231 (2000)
Google Scholar

[ref14] Berlin M., interaction between selenium and inorganicmercury, Environ Health Perspect, 25, 67-69 (1978)
Google Scholar

[ref15] Singh N., Hepatic hydrolytic enzymes following HgCl2intoxication in albino rat, M. Phil. Dissertation, Dr. B. R.Ambekar University, Agra (2003)