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Generation and Characterization of specific Chicken Egg Yolk Antibodies (IgY) against Microbial Bio-terroristic Agent (Vibrio cholerae)

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Author Affiliations

  • 1Department of Microbiology, PSG College of Arts and Science, Coimbatore, TN, INDIA
  • 2Department of Microbiology, PSG College of Arts and Science, Coimbatore, TN, INDIA
  • 3Department of Microbiology, PSG College of Arts and Science, Coimbatore, TN, INDIA

Res. J. Animal, Veterinary and Fishery Sci., Volume 2, Issue (2), Pages 9-12, February,24 (2014)

Abstract

Bioterrorism, the deliberate release of microbial agents or other agents which can cause various illness or death in living things. Egg yolk immunoglobulin (IgY) was prepared against cholera, by immunizing hens with formalin killed V. cholera serotype ogawa. Booster injections of the antigen were subsequently given to the chicken. Using polyethylene glycol and ammonium sulfate precipitation method the antibodies produced were purified. Further Diethylaminoethyl (DEAE) cellulose ion-exchange column chromatography was also carried out. From the egg yolk the IgY fraction was separated and each step of the separation was viewed on Sodium Dodecyl Sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The specificity and reactivity of V. cholera was confirmed by ELISA method. The highest titer of antibody at the dilution of 1:10000 was observed at the 21st week of vaccination and the serum agglutination test also showed a positive result. So it can be suggested that anti- V. cholera produced had a high specificity towards V. cholera O1. As a result anti- V. cholerae IgY may be utilized as preventive agent against Cholera infection.

References

  1. Greenfield Ronald A., Drevets M., Douglas A., Machado.,Linda J., Voskuhl Gene W., Cornea, Pau., Bronze.,Michael S., Bacterial Pathogens as Biological Weapons andAgents of bioterrorism, American Journal of the MedicalSciences, 323(6), 299-315 (2002)
  2. Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J., Protein measurement with the Folin phenol reagent, J BiolChem, 193(1), 265–75 (1951)
  3. Laemmli U.K., Cleavage of structural proteins during theassembly of the head of bacteriophage T4, Nature, 227,680–5 (1970)
  4. Akita E.M. and Nakai S., Comparison of four purificationmethods for the production of immunoglobulins from eggslaid by hens immunized with an enterotoxigenic E.colistrain, Journal of Immunological Methods, 160, 207-214(1993)
  5. Sunwoo H.H., Lee E.N., Mennien K., Suresh M.R. and SimJ.S., Growth inhibitory effect of Chicken egg yolk antibody(IgY) on Escherichia coliO157:H7, J Food Sci, 67, 1486-1494 (2002)
  6. Polson A., Von Wechmar M.B., Van Regenmortel M.H., Isolation of viral IgY antibodies from yolks of immunizedhens, Immunol Commun., 9(5), 475–493 (1980)
  7. Voller A., Bartlett D.E., Bidwell D.E., Clark M.F., AdamsA.N., The detection of viruses by enzyme-linkedimmunosorbent assay (ELISA), J Gen Virol, 33(1), 165–167 (1976)
  8. Kazuyuk, Hirai, Hideyuki Arimitsu, Koji Umeda, KenjiYokota, Lianhua Shen, Kiyoshi Ayada, YoshikatsuKodama, Takao Tsuji, Yoshikazu Hirai, Keiji Oguma, Passive Oral immunization by Egg Yolk Immunoglobulin(IgY) to Vibrio cholera effectively prevents Cholera, ActaMedica Okayama, 64(3), 163-170 (2010)