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Bioassay guided study of the flowers of Hagenia abyssinica against potato wilt disease

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Author Affiliations

  • 1Ethiopian Biotechnology Institute, Addis Ababa, Ethiopia
  • 2Ethiopian institute of Agricultural Research, Addis Ababa, Ethiopia

Res. J. Agriculture & Forestry Sci., Volume 9, Issue (1), Pages 9-14, January,8 (2021)

Abstract

Bacteria wilt disease, which is caused by the bacterium Ralstonia solanacearum, mainly attacks potato, tomato and zinger. In Ethiopia, there is no effective controlling strategy yet. This disease has become the second constraint for potato production next to late blight. Therefore, to develop eco-friendly antibacterial compound, three medicinal plant extracts namely flowers of H. abyssinica, bark of C. macroschyus and fruit of P. dodecandra were screened against this disease. In vitro antibacterial tests of the ethanol extract of H. abyssinica flowers was found to be the most effective of the three plant extracts. A minimum inhibition zone of 20 mm was observed, this being more active than the commercial anti wilt substance known as kocide, which had only 15 mm inhibition zone. Kocide is the commercial anti-wilt preparation which is made up of Cu(OH)2. Five batches of flowers of H. abyssinica were separately extracted with hexane, diethyl ether, ethyl acetate, ethanol and water. Only the ethanol and water extracts were active. From the ethanol extract, the most active anti-wilt substance was isolated and characterized as the known compound quercetin-3-O-glucoside.

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