6th International Young Scientist Congress (IYSC-2020) will be Postponed to 8th and 9th May 2021 Due to COVID-19. 10th International Science Congress (ISC-2020).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Evaluation of mutagenic efficiency and effectiveness of gamma irradiation doses in two cultivars of bread wheat (Triticum aestivum L.)

Author Affiliations

  • 1School of Studies in Botany Vikram University- 456010, Ujjain, MP, India
  • 2School of Studies in Botany Vikram University- 456010, Ujjain, MP, India
  • 3School of Studies in Botany Vikram University- 456010, Ujjain, MP, India
  • 4School of Studies in Botany Vikram University- 456010, Ujjain, MP, India

Res. J. Agriculture & Forestry Sci., Volume 8, Issue (1), Pages 29-33, January,8 (2020)

Abstract

Seed irradiation before sowing processes is one of the most efficient tools to improve the plant genetic diversity rapidly. The present research was carried out to evaluate the mutagenic efficiency and effectiveness of different gamma irradiation doses in bread wheat (Triticum aestivum L.). For this, healthy seeds of two varieties of bread wheat (Lok-1 and WH-147) were treated with various gamma irradiation doses (5, 10, 15, 20, 25, 30, 35, 40, 50kR) at Bhabha Atomic Research Center (BARC), Mumbai. The biological damage was evaluated in M1 populations by collecting the data related to plant lethality, seedling injury, meiotic aberrations and pollen sterility. Biological damage in M1 progenies and chlorophyll mutations in M2 populations were also estimated to measure the mutagenic efficiency and effectiveness of gamma rays. The results obtained showed that mutagenic effectiveness boosted along with increased dose of gamma irradiations whereas the efficiency of gamma ray treatments revealed irregular tendency depending on the biological parameter undertaken for its estimation. The lower as well as intermediate irradiation doses were successfully found to be much effective in producing populations with less genetic damage and with greater amount of macro-mutations. Among the bread wheat cultivars used, var. Lok-1 was found to be more sensitive to gamma-irradiations as compared to WH-147.

References

  1. Khah M.A. and Verma R.C. (2015)., Assessment of the effects of gamma radiations on various morphological and agronomic traits of common wheat (Triticum aestivum L.) var. WH-147., European Journal of Experimental Biology, 5(7), 6-11.
  2. Khah M.A. and Verma R.C. (2017)., Effect of gamma irradiation on seed germination and chromosome behaviour at meiotic division in bread wheat (Triticum aestivum L.)., J. Indian Bot. Soc., 96, 209-215.
  3. Verma R.C. and Shrivastava P. (2014)., Radiation-induced reciprocal translocations in safflower (Carthamus tinctorius L.)., Cytologia, 79(4), 541-545.
  4. Verma R.C. and Khah M.A. (2016)., Assessment of gamma rays induced cytotoxicity in common wheat (Triticum aestivum L.)., Cytologia, 81, 41-45.
  5. Bhatia C. and Swaminathan M.S. (1963)., Frequency and spectrum of mutations induced by radiations in some varieties of bread wheat., Euphytica, 12, 97-112.
  6. Gottschalk W. and Wolff G. (1983)., Induced mutation in plant breeding., Springer Verlag Berlin Heidelberg.
  7. Chopra V. (2005)., Mutagenesis: Investigating the process and processing the outcome for crop improvement., Curr. Sci., 89, 353-359.
  8. Gustaffson A. (1940)., The mutation system of the chlorophyll apparatus., Lund. Univ. Arskr. N.F. Avd., 36, 1- 40.
  9. Konzak C.F., Nilan R.A., Wagner J. and Foster R.J. (1965)., Efficient chemical mutagenesis in the use of induced mutations in plant breeding., Rad. Bot., 5, 49-70.
  10. Shah T.M., Mirza J.I., Haq M.A. and Atta B.M. (2008)., Induced genetic variability in chickpea (Cicer arietinum L.). II. Comparative mutagenic effectiveness and efficiency of physical and chemical mutagens., Pak. J. Bot, 40(2), 605-613.
  11. Girija M. and Dhanavel D. (2009)., Mutagenic effectiveness and efficiency of gamma rays, ethyl methane sulfonate and their combined treatments in cowpea (VignaunguiculataL. Walp)., Global J. Mol. Sci., 4, 68-75.
  12. Shirsat R.K., Mohrir M.N., Kare M.A. and Bhuktar (2010)., Induced mutations in horsegram: Mutagenic efficiency and effectiveness., Res. Sci. Technol., 2, 20-23.
  13. Wani M.R., Khan S. and Kozgar M.I. (2011)., Induced chlorophyll mutations. I. Mutagenic effectiveness and efficiency of EMS, HZ and SA in mungbean., Frontiers of Agriculture in China, 5(4), 514-518.
  14. Giri S.P. and Apparao B.J. (2012)., Studies on effectiveness and efficiency of EMS in pigeonpea (CajanuscajanL.)., Bioscience Discovery, 2, 29-31.
  15. Mahamune S.E. and Kothekar V.S. (2012)., Induced mutagenic frequency and spectrum of chlorophyll mutants in French bean., Int. Multid. Res. J., 2, 30-32.
  16. Bashir S., Wani A.A. and Nawchoo I.A. (2013)., Studies on mutagenic effectiveness and efficiency in Fenugreek (Trigonellafoenum-graecum L.)., Afr. J.Biot., 12(18), 2437-2440.
  17. Khah M.A. and Verma R.C. (2017)., Cytological characterization of induced multiple translocation heterozygote in pearl millet (Pennisetum glaucum L.)., Cytologia, 82(4), 443-447.