Bioefficacy and Progeny Production of native new-to-science species of entomopathogenic nematodes, Steinernema dharanaii (TFRIEPN-15) against forest insect pest, Albizia defoliator, Spirama retorta Cramer (Lepidoptera: Noctuidae)
- 1Northern Regional Centre, Zoological Survey of India, 218, Kaulagarh Road, Deharadun-248 195, Uttarakhand, India
- 2Forest Entomology Division, Tropical Forest Research Institute, P. O. RFRC, Mandla Road, Jabalpur- 482021, Madhya Pradesh, India and Institute of Forest Productivity (IFP) Gumla, National Highway-23, Lalgutwa, Ranchi – 835303, Jharkhand, India
Res. J. Agriculture & Forestry Sci., Volume 7, Issue (4), Pages 10-16, October,8 (2019)
The laboratory study examined bioefficacy and progeny production of locally isolated new –to science species of entomopathogenic nematode (EPNTFRI-15), Steinernema dharanaii against forest insect pest, Albizia defoliator, Spirama reortra Cram. (Lepidoptera: Noctuidae) in the laboratory. The larvae were exposed to ten dosages viz., 3, 5, 10, 15, 20, 30, 40, 50, 100 and 200IJs larva-1 plate. This EPN species for different time periods and they were found to be susceptible to all the doses tested in filter paper bioassay. However, the susceptibility and progeny production of larvae to nematode infection varied according to the dosages of IJs and their exposure periods. The doses of 3IJs Larva-1 exhibited negligible but statistically significant mortality (17.14%) over untreated control (P<0.05).There were significantly superior (P<0.05) mortalities 74.29%, 100.0% and recorded at 50, 100 and 200IJs Larva-1. The progeny production data from the larvae exposed to different doses of IJs indicated progeny production to be proportional to the IJ doses up to 100IJs Larva-1 at which it was significantly higher 37,400 IJs (P<0.05), which decreased with increase in IJ doses. However, progeny production at the highest dose of 200 IJs Larva-1 (18,180IJs) was still significantly superior over IJs obtained at the lowest dose (P<0.05). The probit analysis performed, indicated 7.07 (UL 11.47 and LL 4.37IJs Larva-1) and 34.67IJs Larva-1 (UL 51.32 and LL 23.42IJs Larva-1) were required to cause, respectively 50 and 90.0% mortality in albizia defoliator larvae in laboratory (P<0.05) . At the same time 34.51 (UL 80.60 and LL 14.42 hrs) and 131.82 hrs (UL 218.98 and LL 79.35hrs) were required for causing 50 and 90.0% mortality, respectively. This locally isolated new-to-science EPN species, Steinernmea dharanaii thus have potential scope for the management of major insect pests of forestry and agricultural importance in India.
- Hokkanen HMT. (2015)., Integrated pest management at the crossroads: Science, politics, or business (as usual)?., Arthropod-Plant Inte, 9, 543-545.
- Kulkarni N. (2014)., Status of potential of biocontrol component for integrated management of forest insect pests in India., In Biopesticides in Sustainable Agriculture: Progress and Potential, O. Koul, G.S. Dhaliwal, S. Khokar, and R. Singh, eds.), Science Publisher, New Delhi, India, 389-419.
- Kulkarni N. (2017)., 16 Integrated Insect Pest Management in Tropical Forestry., Integrated Pest Management in Tropical Regions, 313-342.
- Poinar G. (1990)., 0. Jr. 1979. Nematodes for biological control of insects., Boca Raton, FL, USA, CRC Press, 227.
- Gaugler R. (1981)., Biological control potential of neoaplectanid nematodes., J. Nematolo., 13, 241-249.
- Grewal P.S. and Peters A. (2005)., Formulation and quality. Nematodes as biocontrol agents., CABI Publishing, Wallingford, 79-90.
- Hussaini S.S., Rabindra R.J. and Nagesh M. (2003)., Current Status of Research on Entomopathogenic Nematodes in India., Project Directorate of Biological Control, PDBC, Bangalore, India, 218.
- Grewal P.S., Ehlers R-U. and Shapiro-Ilan D.I. (2005)., Nematodes as biocontrol agents., New York: CABI Publishing.
- Bedding R.A. (2006)., Entomopathogenic nematodes from discovery to application., Biopesticides International, 2, 87-119.
- Kulkarni N., Hussaini S.S., Paunikar S. and Joshi K.C. (2008)., Entomopathogenic Nematodes in insect pest management of forestry and plantation crops: An appraisal., Indian J. Tropical Biodiversity, 16(2), 156-165.
- Susurluk I.A., Kumral N.A., Bilgili U. and Acikgoz E. (2011)., Control of a new turf pest, Dorcadion pseudopreissi (Coleoptera: Cerambycidae), by the entomopathogenic nematode Heterorhabditis bacteriophora., J. Pest Sci., 84(3), 321-326.
- Ulu T.C., Sadic B., Susurluk I.A. and Aksit T. (2015)., Virulence of four entomopathogenic nematode species for plum sawfly, Hoplocampa flava L. (Hymenoptera: Tenthredinidae)., Invertebrate Survival Journal, 12(1), 274-277.
- Paunikar S. and Kulkarni N. (2018)., Evaluation of entomopathogenic nematode Steinernema dharanaii NR (Tfriepn-15) on greater wax moth, Galleria Mellonella., Indian Journal of Entomology, 80(4), 1506-1512.
- Boemare N., Laumond C. and Mauleon H. (1996)., The entomopathogenic nematode-bacterium complex: biology, life cycle and vertebrate safety., Biocontrol Science and Technology, 6(3), 333-346.
- Ehlers R.U. (1996)., Current and future use of nematodes in biocontrol: practice and commercial aspects with regard to regulatory policy issues., Biocontrol Science and Technology, 6(3), 303-316.
- Ehlers R.U. (2001)., Mass production of entomopathogenic nematodes for plant protection., Applied microbiology and biotechnology, 56(5-6), 623-633.
- Kaya H.K. and Gaugler R. (1993)., Entomopathogenic nematodes., Annual review of entomology, 38(1), 181-206.
- Hazir S., Kaya H.K., Stock S.P. and Keskin N. (2004)., Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) for biological control of soil pests., Turkish Journal of Biology, 27(4), 181-202.
- Akhurst R.J. and Boemare N.E. (1990)., Biology and Taxonomy of Xenorhabdus., Gaugler R, Kaya HK (eds), Entomopathogenic Nematodes in Biological Control, CRC Press, Boca Raton, FL, 75-90.
- Brown I.M. and Gaugler R. (1997)., Temperature and humidity influence emergence and survival of entomopathogenic nematodes., Nematologica, 43(5), 363-375.
- Grewal P. and Georgis R. (1999)., Entomopathogenic nematodes., In Biopesticides: use and delivery, 271-299. Humana Press.
- Bhasin G.D. and Roonwal M.L. (1954)., A List of Insect Pests of Forest Plants in India and the Adjacent Countries. Part 2. List of insect pests of plant genera, A List of Insect Pests of Forest Plants in India and the Adjacent Countries. Part 2. List of insect pests of plant genera
- Beeson C.F.C. (1941)., The ecology and control of the forest insects of India and the neighbouring countries., Publishers Vasant Press, Dehradun, 1007.
- Lefroy H.M. (1909)., Indian Insect Life., Reprint edition 1971, Today & Tomorrows Printers & Pubilcation, New Delhi, 786.
- Sen-Sarma P.K. and Thakur M.L. (1985)., Pest management in Indian forestry., Indian Forester, 111(11), 956-964.
- Sambath S., Humane S., Kiran U., Roychoudhury N. and Joshi K.C. (1993)., Insect pests of Albizia lebbeck Linn and Albizia procera (Roxb.) Benth., In : Proceedings of Reigional Meeting of Silviculturisist and Research Workers of Central Region, held on 19-20 December 1992., Chandrapur , Maharashtra, 265-270.
- Sambath S. and Joshi K.C. (2004)., Bionomics of Spirama retorta Cram. (Lepidoptera: Noctuidae)., Indian J. Entomol., 66, 102-106.
- Sambath S. and Joshi K.C. (1999)., Relative toxicity of some insecticides tested against the larvae of Albizia foliage feeder., Spirama retorta. Indian J. Entomol., 61, 275-278.
- Roychoudhury N. and Joshi K.C. (2006)., Relative resistance in Albizia procera (Roxb.) Benth., against foliage feeder, Spirama retorta Cramer, due to seed source variation and role of leaf water., J. Trop. Forestry, 22, 43-48.
- Roychoudhury N. and Joshi K.C. (2011)., Effect of ivermectin on larval mortality and reproductive potential of Albizia foliage feeder, Spirama retorta Cramer (Lepidoptera: Noctuidae)., J. Pest Science, 84, 117-122.
- Paunikar S., Kulkarni N., Mishra V. Tiple A., Hussaini S.S. (2010)., Susceptibility of, Teak Skeletonizer, Eutectona machaeralis Walker to Steinernema carpocapsae., Hislopia J., 1(2), 165-170.
- Kulkarni N., Paunikar S. and Mishra V. (2011)., Pathogenicity of entomopathogenic nematode, Heterorhabditis indica Poinar against the defoliator, Spirama retorta Cramer (Lepidoptera: Noctuidae) Insect Pest Management, A current Scenario, 2011., (Eds. Dunston P. Ambrose), Entomology Research Unit, St. Xavier's College Palayamkottai India, 312-115.
- Paunikar S., Mishra V., Kulkarni N. and Bhandari R. (2011)., Virulence of entomopathogenic nematodes, Heterorhabditis indica against the teak defoliator, Hyblaea purea Cramer (Lepidoptera: Hyblaeidae)., Paper presented at National Seminar on Progress in Life Sciences for welfare of Human″ 5th-6th February 2011 at Govt. Model Science College (Autonomous), Jabalpur, 38.
- Kulkarni N., Paunikar S., Mishra V.K., Daksh S. and Hussaini S.S. (2013)., Efficacy of entomopathogenic nematodes, Heterorhabditis indica against the Bamboo leaf roller Crypsiptya coclesalis Walker (Lepidoptera : Pyralidae)., Paper presented in International Conference on insect Science, (February 14th-17th 2013) Bangalore, University of Agriculture sciences India, 21.
- Kulkarni N., Mishra V.K. and Paunikar S.D. (2017)., Infectivity of native populations of entomopathogenic nematodes against teak defoliators., J. Entomol. and Zool.Stud., 5(6), 639-643.
- Kulkarni N., Rizvi A., Kumar V., Paunikar S. and Mishra V.K. (2012)., Morphological and molecular characterization of Steinernema dharanaii sp. N. (Nematoda: Steinernematidae): An Entomopathogenic Nematode from India., Indian J. Tropical Biodiversity, 20(2), 107-116.
- Bedding R.A. and Akhurst R.J. (1975)., A simple technique for the detection of insect parasitic rhabditid nematodes in soil., Nematologica, 21, 109-116.
- Woodring J.L. and Kaya H.K. (1988)., Steinernematid and heterorhabditid nematodes: a handbook of techniques southern cooperative series bulletin 331., Arkansas Agricultural Experiment Station, Fayatteville.
- Kaya H.K. and Stock S.P. (1997)., Techniques in insect nematology., In L. A. Lacey (Ed.), Manual of Techniques in Insect Pathology. Academic Press, London, 282-324.
- Gomez K.A. and Gomez A.A. (1994)., Statistical procedures for Agricultural Research (Second Edition)., an International Rice Research Institute Book. A Wiley International Publication, John Wiley & Sons, New York, 680.
- Busvine J.R. (1971)., A Critical Review on the Techniques for Testing Insecticides., Commonwealth Agricultural Bureaux Slough, England, 345.
- Finney D.J. (1971)., Probit analysis., Cambridge University Press: Cambridge, UK, 333.
- Shapiro-Ilan D., Han R. and Qiu X. (2014)., Production of entomopathogenic nematodes. In Mass Production of Beneficial Organisms., Invertebrates and Entomopathogens (Ramos, J.M., Rojas, M.G., Shapiro-Ilan, D.I. eds.), Elsevier Inc., USA, 321-355.
- Kulkarni N., Paunikar S., Hussaini S.S. and Joshi K.C. (2011)., Susceptibility of teak skeletonizer, Eutectona machaeralis Walker to EPN., Heterorhabditis indica Poinar. World J. Zool., 6(1), 33-39.
- Paunikar S. and Kulkarni N. (2018)., Evaluation of native new-to-science species of entomopathogenic nematode, Steinernema dharanaii, (TFRIEPN-15) against Bamboo leaf roller, Crypsipyta coclesalis Walker (Lepidoptera: Pyralidae) in the laboratory., The Indian Forester (Communicated).