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Morphological and metabolic characterization of wilt disease (fusarium oxysporum f. sp. ciceri) in chickpea (Cicer arietinum L.)

Author Affiliations

  • 1Department of Biotechnology, Junagadh agricultural University, Junagadh, Gujarat-362001, India
  • 2Department of Biotechnology, Junagadh agricultural University, Junagadh, Gujarat-362001, India
  • 3Department of Biotechnology, Junagadh agricultural University, Junagadh, Gujarat-362001, India
  • 4Department of Biotechnology, Junagadh agricultural University, Junagadh, Gujarat-362001, India
  • 5Department of Biotechnology, Junagadh agricultural University, Junagadh, Gujarat-362001, India
  • 6Department of Biotechnology, Junagadh agricultural University, Junagadh, Gujarat-362001, India
  • 7Department of Biotechnology, Junagadh agricultural University, Junagadh, Gujarat-362001, India
  • 8Department of Biotechnology, Junagadh agricultural University, Junagadh, Gujarat-362001, India
  • 9Department of Biotechnology, Junagadh agricultural University, Junagadh, Gujarat-362001, India

Res. J. Agriculture & Forestry Sci., Volume 5, Issue (7), Pages 12-19, July,8 (2017)

Abstract

Wilt disease of chickpea caused by the diminish pathogen Fusarium oxysporum f. sp. ciceri (foc) is a serious disease of chickpea (Cicer arietinum L.) and it cause approximately 10-15% loss in annual yield. This phytopathogenic fungus invades the plant via roots, colonizes the xylem vessels and prevents the nutrient flow, finally resulting in wilting of the whole plant thus it is also known as vascular wilt. For the experiment the wilt causing fungi has been isolated from recently wilted wilt susceptible chickpea (JG-62). From the damaged root part, 4 isolates of F. oxysporum were purified. The purified colony of F. oxysporum ranged diameter of 3.0 - 4.0 cm PDA medium at fourth day of inoculation. The mycelia characters had white to creamy and tinge pink, margins slightly lobed or smooth on PDA medium. This identification was further confirmed by Microcopy, Scanning electron microscopy (SEM) and 18s rRNA Sequencing of ITS region of Fungi. Dendogram created from sequences represents the strain isolated from wilt sick plot is highly similar to Fusarium oxysporum. Further, we carried out metabolic profiling of foc in GC-MS. The polar and non polar metabolic phase was separated. We identified total 49 metabolites in foc among them, the higher intensity of sugar, sugar alcohols, amino acids and fatty acid esters was found. This study can provide metabolomic and morphological study to understand pathogenesity of chickpea wilt.

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