6th International Young Scientist Congress (IYSC-2020) will be Postponed to 8th and 9th May 2021 Due to COVID-19. 10th International Science Congress (ISC-2020).  International E-publication: Publish Projects, Dissertation, Theses, Books, Souvenir, Conference Proceeding with ISBN.  International E-Bulletin: Information/News regarding: Academics and Research

Cellulase Production by Utilizing Agricultural Wastes

Author Affiliations

  • 1Post Graduate Department of Microbiology, Yashwant Mahavidhyalaya, Nanded, MS, INDIA
  • 2Post Graduate Department of Microbiology, Yashwant Mahavidhyalaya, Nanded, MS, INDIA
  • 3Post Graduate Department of Microbiology, Yashwant Mahavidhyalaya, Nanded, MS, INDIA
  • 4Post Graduate Department of Microbiology, Yashwant Mahavidhyalaya, Nanded, MS, INDIA
  • 5Post Graduate Department of Microbiology, Yashwant Mahavidhyalaya, Nanded, MS, INDIA

Res. J. Agriculture & Forestry Sci., Volume 1, Issue (7), Pages 6-9, August,8 (2013)

Abstract

Cellulases are the group of hydrolytic enzymes and they are capable of degrading all types of lignocellulosic materials. Cellulases have wide range of applications. Present work focuses on the factors for improvement of enzymatic hydrolysis of various agricultural wasets by using different fungal species. It was the aim to study the Cellulase enzyme production ability of fungal strains against the lignocellulosic bio-waste like Rice husks, Millet husks, Banana peels, Wheat bran, Coir waste and saw dust. Simultaneously the effect of different environmental factors such as pH (3-9), substrate concentration (1%-6%) and temperature (10C-50C). The enzyme assay was carried out by two methods CMCase and FPase and the production was analyzed individually by Miller's modified method of Dinitrosalicylic acid (DNSA). In this study the high level of enzyme production was obtained at 30C and 40C by using Rice husks, Banana peels, Millet husks, Wheat bran, saw dust and Coir waste by using varying substrate concentrations. Out of all the six used substrates banana peels gave the higher production of the cellulase enzyme that is 12.4 units / ml after 4 days of incubation.

References

  1. Immanuel G., Akila Bhagwat C.M., Iyappa Raj P., Esakkiraj P.and Palavesam A., Production and Partial purification ofCellulase by Aspergillus niger and A. fumigates fermented incoir waste and sawdust, International J. of Microbiol, (2009)
  2. Shin C.S., Lee J.P., lee I.S. and Park S.C., Enzyme productionof Trichoderma ressei, Rut C-30 on various lignocellulosicsubstrates, Appl. Biochem. Biotech., 84-86 (1-9), 237245(2000)
  3. Fadel M., Production physiology of Cellulase and -glucosidase enzyme of Aspergillus niger grown under solidstate fermentation conditions, Biological Sci., 1(5), 401411(2000)
  4. Wyman C.E., biomass Ethanol: Technical progress, opportunities, and commercial challenges, Annu. Rev. enrgyEnviron., 24, 189-226 (1999)
  5. Zhang Y.H.P. and Lynd L.R., Toward an aggregatedunderstanding of enzymatic hydrolysis of cellulose: Noncomplexedcellulase systems, Bitechnol. Bioeng., 89, 797-824(2004)
  6. Demain A.L., Newcomb M. and J. H. D. Wu, Cellulase,clostridia and ethanol, Microbiol. Mol. Bio. Rev., 69, 124154(2005)
  7. Ali S., Sayed A., Sarker R.T., and Alau R., Factors affectingcellulase production by Aspergillus niger and Aspergillusterrus using water Hyacinth, World J. of MicrobialBiotechnology, 7, 6266 (1991)
  8. Gharpuray M. M., Lee Y.H., Fan L.T., Structural modificationof lignocellulosic by treatment to enhance enzymatichydrolysis, Biotechnol., 25, 157170 (1983) Wang C.T., Hseu and Huang C., Introduction of Cellulase bycello-oligosaccharides in Trichoderma konigii G-39, J.Biotechnol., 9, 47-60 (1988)
  9. Gadgil N.J., Daginawala HF, Chakarabarti T, and Khanna P., Enhanced cellulase production by a mutant of Trichodermareesei., Enzyme Microb Technol., 17, 942-946 (1995)
  10. Miller G.L., Use of Dinitrosalicylic acid reagent fordetermination of reducing sugar, Biotechnol. Bioeng. Symp., 5,193-219 (1972)
  11. Milala M.A., Shugaba A., Gidado A., Ene A. C. and WafarJ.A., Studies on the use of agricultural wastes for cellulaseenzyme production by Aspergillus niger, Res. J. of Agri. Andbiology. Sci., 1(4), 325-328 (2005)
  12. Gbekeloluwa B.O. and Moo-young
  13. -glucosidase by Neurospora sitophila, World J. ofMicrobial Biotechnol., 7, 411 (1991)